Calnexin, VEGFA, CD31, HIF-1 alpha, (AB0037, AB0063, AB0092, AB0112, Sicgen, Portugal), β-actin, α-tubulin (A5316, T6199, Sigma, USA), PPARγ, AKT, p-AKT(Ser473), VEGFR2, PI3K (#2443, #9272, #4058, #2479, #4249, Cell Signaling, USA), ANG-2, F4/80, Perilipin-A, GLUT4, p-IR(Y1361), GLO-I, C/EBPalpha, PGC1alpha, ANGPTL4, AT1, HIF-2 alpha (Ab8452, Ab74383, Ab3526, Ab65267, Ab60946, Ab96032, Ab40764, Ab191838, Ab2920, Ab9391, Ab8365, Abcam, UK), CD11c, CD206 (bs-2058R, bs-2664R Bioss, USA) TIE-2, IRβ (sc-324, sc-57342, SantaCruz Biotechnology, USA), CEL (KH025, TransGenic Inc, Japan).
Ab2920
Manufactured by Abcam
Sourced in United Kingdom
Ab2920 is a lab equipment product from Abcam. It is a core piece of equipment used for analysis and experimentation in various research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab40764, by Abcam (1 mentions)
Ab8365, by Abcam (1 mentions)
Ab191838, by Abcam (1 mentions)
Ab9391, by Abcam (1 mentions)
Ab8452, by Abcam (1 mentions)
Ab65267, by Abcam (1 mentions)
Ab96032, by Abcam (1 mentions)
A5316, by Merck Group (1 mentions)
Ab74383, by Abcam (1 mentions)
Ab60946, by Abcam (1 mentions)
Ab3526, by Abcam (1 mentions)
T6199, by Merck Group (1 mentions)
Vegfr2, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Ponceau s red, by Merck Group (1 mentions)
Ecl prime, by GE Healthcare (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
2 protocols using ab2920
1
Comprehensive Protein Expression Analysis
2
Western Blot Analysis of ANGPTL-4
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Plasma samples (7-10 µg proteins) were loaded in Laemmli buffer and size separated by SDS-PAGE. After protein transfer to PVDF membranes (GE Healthcare) and blocking with 5% nonfat dried milk/Tween-PBS (1 h), a commercially available rabbit polyclonal antibody validated for Western blotting and directed against the N-terminal domain of ANGPTL-4 (1:1000; Abcam ab2920), which is the domain interacting with LPL (Sukonina et al. 2006) (link), was applied overnight at 4°C. After washing, an IgG-peroxidase-conjugated secondary antibody was applied for 1 h (1:5000). Blots were incubated in chemiluminescence reagents (ECL Prime, GE Healthcare) and bands were detected using the ChemiDoc XRS+ Imaging System (BioRad). ANGPTL-4 values were normalized to serum albumin levels (Ponceau S Red; Sigma). Values are expressed as percent of the saline group.
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