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Kingfisher duo machine

Manufactured by Thermo Fisher Scientific
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The KingFisher Duo is a compact automated sample preparation instrument designed for a wide range of applications, including nucleic acid purification, protein purification, and sample handling. The instrument utilizes magnetic particle separation technology to efficiently isolate and purify target analytes from various sample types.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (5 mentions) 2100 bioanalyzer instrument, by Agilent Technologies (4 mentions) Mag jet rna kit, by Thermo Fisher Scientific (4 mentions) Ncounter gene expression assay, by NanoString (4 mentions) Nsolver software, by NanoString (4 mentions)

4 protocols using kingfisher duo machine

1

Transcription Factor Expression Analysis

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RNA was extracted separately from snap-frozen DD cords and nodules from the original cohort of 6 patients used for DAB immunohistochemical staining, and was used for NanoString nCounter Gene Expression Assay (Nanostring Technologies, Seattle, Wash.). Total RNA was extracted using the MagJET RNA kit (Thermo Fisher Scientific) with the protocol adapted for tissue, and run on a KingFisher Duo machine (Thermo Fisher Scientific). RNA samples were then quantitated on a Qubit 2.0 fluorometer (Life Technologies) and were subjected to RNA integrity analysis using the 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, Calif.). Samples then underwent NanoString nCounter Gene Expression Assay performed by New Zealand Genomics (Dunedin, New Zealand) according to the manufacturer’s protocol. Probes for the genes encoding NANOG (NM_024865.2), SALL4 (NM_020436.3), SOX2 (NM_003106.2), OCT4 (NM_002701.4), and STAT3 (NM_139276.2) and the housekeeping gene GUSB (NM_000181.1) were designed and manufactured by NanoString Technologies. Raw data was analyzed using nSolver software (NanoString Technologies) using standard settings and normalized against the housekeeping gene.
Koh S.P., On N., Brasch H.D., Chibnall A.M., Armstrong J.R., Davis P.F., Tan S.T, & Itinteang T. (2016). Embryonic Stem Cell–like Population in Dupuytren’s Disease. Plastic and Reconstructive Surgery Global Open, 4(11), e1064.
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2

Quantifying Stem Cell Markers in MDLSCC

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Total RNA was isolated and quantified as previously described (31 (link)) from six snap-frozen MDLSCC samples from the original cohort of 10 patients used for DAB IHC staining. They were subjected to the NanoString nCounter gene expression assay (NanoString Technologies, Seattle, WA, USA) by New Zealand Genomics (Dunedin, New Zealand). Briefly, total RNA was extracted using the MagJET RNA kit (Thermo Fisher Scientific) with the protocol adapted for tissue and run on a KingFisher Duo machine (Thermo Fisher Scientific). RNA samples were then quantitated on a Qubit® 2.0 fluorometer (Thermo Fisher Scientific) and were subject to RNA integrity analysis via the 2100 Bioanalyzer Instrument (Agilent Technologies). Probes for the genes encoding CD44 (NM_001001392.1), NANOG (NM_024865.2), OCT4 (NM_002701.4), STAT3 (NM_139276.2), and the housekeeping genes glucuronidase beta (GUSB) (NM_000181.1), clathrin heavy chain (CLTC) (NM_4859.2), and hypoxanthine phosphoribosyltransferase 1 (NM_000194.1) were designed and manufactured by NanoString Technologies. Raw data were analyzed using nSolver™ software (NanoString Technologies) using standard settings and normalized against the housekeeping gene.
Ram R., Brasch H.D., Dunne J.C., Davis P.F., Tan S.T, & Itinteang T. (2017). The Identification of Three Cancer Stem Cell Subpopulations within Moderately Differentiated Lip Squamous Cell Carcinoma. Frontiers in Surgery, 4, 12.
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3

Quantification of Pluripotency Genes in CAML

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RNA was extracted from six snap-frozen samples of CAML from the same cohort of nine patients used for DAB IHC staining, was analyzed using NanoString nCounter™ Gene Expression Assay (NanoString Technologies, Seattle, WA, USA). Total RNA was extracted using the MagJET RNA kit (Thermo Fisher Scientific) with the protocol adapted for tissue, and run on a KingFisher Duo machine (Thermo Fisher Scientific). The RNA samples were then quantitated on a Qubit® 2.0 fluorometer (Invitrogen, Life Technologies) and were subject to RNA integrity analysis via the 2100 Bioanalyzer Instrument (Agilent Technologies). The samples then underwent NanoString nCounter gene expression assay performed by New Zealand Genomics (Dunedin, New Zealand) according to the manufacturer’s protocol. Probes for the genes encoding OCT4 (POU5F1, NM_002701.4), SOX2 (NM_003106.2), NANOG (NM_024865.2), KLF4 (NM_004235.4), c-Myc (NM_002467.3), and the housekeeping gene GUSB (NM_000181.1) were designed and synthesized by NanoString Technologies. Raw data were analyzed using nSolver™ software (NanoString Technologies) using standard settings and normalized against the housekeeping gene.
Humphries H.N., Wickremesekera S.K., Marsh R.W., Brasch H.D., Mehrotra S., Tan S.T, & Itinteang T. (2018). Characterization of Cancer Stem Cells in Colon Adenocarcinoma Metastasis to the Liver. Frontiers in Surgery, 4, 76.
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4

Quantitative Gene Expression Analysis of Stem Cell Markers

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RNA was extracted from five snap-frozen MDBMSCC samples from the same cohort of patients used for DAB IHC staining and was used for NanoString nCounter™ Gene Expression Assay (Nanostring Technologies, Seattle, WA, USA). Total RNA was extracted using the MagJET RNA kit (Thermo Fisher Scientific) with the protocol adapted for tissue and run on a KingFisher Duo machine (Thermo Fisher Scientific). RNA samples were then quantitated on a Qubit® 2.0 fluorometer (Invitrogen, Life Technologies) and were subject to RNA integrity analysis via the 2100 Bioanalyzer Instrument (Agilent Technologies). The samples then underwent NanoString nCounter gene expression assay performed by New Zealand Genomics (Dunedin, New Zealand) according to the manufacturer’s protocol. Probes for the genes encoding NANOG (NM_024865.2), SALL4 (NM_020436.3) SOX2 (NM_003106.2), OCT4 (NM_002701.4), CD44 (NM_001001392.1), and STAT3 (NM_139276.2) and the housekeeping gene GUSB (NM_000181.1) were designed and manufactured by NanoString Technologies. Raw data were analyzed using nSolver™ software (NanoString Technologies) using standard settings and normalized against the housekeeping gene.
Yu H.H., Featherston T., Tan S.T., Chibnall A.M., Brasch H.D., Davis P.F, & Itinteang T. (2016). Characterization of Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma. Frontiers in Surgery, 3, 46.
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