Silver perchlorate monohydrate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Silver perchlorate monohydrate is a chemical compound with the molecular formula AgClO4·H2O. It is a crystalline solid that is soluble in water and organic solvents. Silver perchlorate monohydrate is commonly used as a laboratory reagent and in certain analytical applications.

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4 protocols using silver perchlorate monohydrate

Synthesis and Characterization of Silver Nanoparticles

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Silver nitrate (99.9%), sodium citrate dehydrate (99%), sodium borohydride (98%), hydrogen peroxide (30%), polyvinylpyrrolidone (PVP), 2-mercaptoethanol (99%), 11-amino-1-undecanethiol hydrochloride (MUNH₂, 99%), ofloxacin powder (99%), orthovanadate (≥90%), sodium chloride, sodium phosphate, sodium phosphate monobasic monohydrate, Bacto-Tryptone, and Bacto Yeast Extract were purchased from Sigma-Aldrich. N-Hydroxysulfosuccinimide (Sulfo-NHS, 98.5%, Pierce), 1-ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC, 99%, Pierce), silver perchlorate monohydrate (99%, Alfa Aesar), live/dead backlight viability assay (Life Technologies), and Hoechst 33342 (Life Technologies) were purchased as indicated, and used as received. The cell line of Escherichia coli (wt w3110) was purchased from a genetic stock center (CGSC). Deionized (DI) water (18 MΩ water, Barnstead) was used to prepare all the solutions including a standard LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.5% NaCl, pH = 7.2) and a modified LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.1% NaCl, pH = 7.2).

Songkiatisak P., Ding F., Cherukuri P.K, & Xu X.H. (2020). Size-dependent inhibitory effects of antibiotic nanocarriers on filamentation of E. coli. Nanoscale Advances, 2(5), 2135-2145.

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Synthesis of Silver Oxalate Complex

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Silver perchlorate monohydrate (AgClO₄ ∙ H₂O, 99%, Alfa Aesar, Ward Hill, MA, USA) and potassium oxalate (K₂C₂O₄, special purity grade, 99.9%, Reakhim, Moscow, Russia) were used. Double-distilled water was used as a solvent.

Ershov V., Tarasova N, & Ershov B. (2021). Evolution of Electronic State and Properties of Silver Nanoparticles during Their Formation in Aqueous Solution. International Journal of Molecular Sciences, 22(19), 10673.

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Bacterial Nanoparticle Synthesis Protocol

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Silver nitrate (99.9%), sodium citrate dehydrate (99%), sodium borohydride (98%), hydrogen peroxide (30%), polyvinylpyrrolidone (PVP), 2-mercaptoethanol (99%), 11-amino-1-undecanethiol hydrochloride (MUNH₂, 99%), ofloxacin powder (99%), orthovanadate (≥ 90%,), sodium chloride, sodium phosphate, sodium phosphate monobasic monohydrate, bacto-tryptone, and bacto yeast extract were purchased from Sigma-Aldrich. N-hydroxysulfosuccinimide (Sulfo-NHS, 98.5%, Pierce), 1-Ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC, 99%, Pierce), silver perchlorate monohydrate (99%, Alfa Aesar), Live/dead backlight viability assay (Life Technologies), and Hoechst 33342 (Life Technologies) were purchased as indicated, and used as received. Cell line of Escherichia coli (wt w3110) was purchased from Genetic Stock Center (CGSC). Deionized (DI) water (18 MΩ water, Barnstead) was used to prepare all solutions including standard LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.5% NaCl, pH = 7.2) and modified LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.1% NaCl, pH = 7.2).

Songkiatisak P., Ding F., Cherukuri P.K, & Xu X.H. (2020). Size-dependent inhibitory effects of antibiotic nanocarriers on filamentation of E. coli. Nanoscale Advances, 2(5), 2135-2145.

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Synthesis and Characterization of Silver Nanoparticles

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We purchased silver perchlorate
monohydrate (99%, Alfa Aesar), sodium citrate dihydrate (99%, Sigma),
sodium borohydride (98%, Sigma), hydrogen peroxide (30%, Sigma), polyvinylpyrrolidone
(PVP, Sigma), 2-mercaptoethanol (99%, Sigma), 11-amino-1-undecanethiol
hydrochloride (AUT, 99%, Sigma), 1-ethyl-3-[3-(dimethylamino)propyl]-carbodiimide
hydrochloride (EDC, 99%, Pierce), N-hydroxysulfosuccinimide
(sulfo-NHS, 98.5%, Pierce), ofloxacin (≥98%, Oflx, Enzo), L-broth
(LB) powder (Sigma), and the LIVE/DEAD BacLight viability
and counting assay (Invitrogen) and used them as received. We used
nanopure deionized (DI) water (18 MΩ water, Barnstead) to prepare
commonly used standard LB medium (1% tryptone, 0.5% yeast extract,
and 0.5% NaCl in DI water, pH = 7.2) and our modified LB medium (1%
tryptone, 0.5% yeast extract, and 0.1% NaCl in DI water, pH = 7.2)

Ding F., Songkiatisak P., Cherukuri P.K., Huang T, & Xu X.H. (2018). Size-Dependent Inhibitory Effects of Antibiotic Drug Nanocarriers against Pseudomonas aeruginosa. ACS Omega, 3(1), 1231-1243.

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