We purchased dioctyl phenyl-phosphonate (DOPP), polyvinyl chloride (PVC), 2-hydroxybenzoic acid (2-HBA) and 3-trimethylsilyl-1-propanesulfonic acid- (DSS-d6) from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Tetradodecylammonium bromide (TDAB), 3,5-dihydroxybenzoic acid (3,5-DHBA), aniline and resorcinol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Caffeine was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 2,6-Dihydroxybenzoic acid (2,6-DHBA), tartaric acid and potassium chloride (KCl) were acquired from Kanto Chemical Co., Inc. (Tokyo, Japan). Deuterium oxide (D2O, 99.8 atom% D) was acquired from Acros Organics (Fair Lawn, NJ, USA). Supplementary Figure S1 shows the chemical structures of Caffeine, HBAs, aniline, and resorcinol used in this study.
Caffeine
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Caffeine is a crystalline solid compound that is commonly used as a stimulant in laboratory settings. It has the chemical formula C8H10N4O2 and is classified as a methylxanthine. Caffeine's core function is to act as a central nervous system (CNS) stimulant, which can temporarily increase alertness and energy levels.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Fujifilm (3 mentions)
Sorbitol, by Fujifilm (2 mentions)
Nourseothricin, by Jena Biosciences (2 mentions)
Dithiothreitol (dtt), by Fujifilm (2 mentions)
Hygromycin b, by Tokyo Chemical Industry (2 mentions)
Congo red, by Merck Group (2 mentions)
Tunicamycin, by Cayman Chemical (2 mentions)
3 trimethylsilyl 1 propanesulfonic acid d6 dss d6, by Fujifilm (1 mentions)
Resorcinol, by Merck Group (1 mentions)
Oleic acid, by Fujifilm (1 mentions)
Sodium dihydrogen phosphate dihydrate, by Fujifilm (1 mentions)
8n naoh, by Fujifilm (1 mentions)
Voriconazole, by Tokyo Chemical Industry (1 mentions)
Sodium dodecyl sulfate (sds), by Fujifilm (1 mentions)
Human serum, by Merck Group (1 mentions)
Cacl2, by Kanto Chemical (1 mentions)
Lithium chloride (licl), by Fujifilm (1 mentions)
Fluconazole, by Tokyo Chemical Industry (1 mentions)
Menadione, by Merck Group (1 mentions)
Potassium chloride (kcl), by Fujifilm (1 mentions)
6 protocols using caffeine
1
Characterization of Aromatic Compounds
2
Enzymatic Antioxidant Activity Assay
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Cu/Zn-SOD was purchased
from Doulai Biotechnology Co., Ltd. Caffeine (98% purity) was purchased
from Tokyo Chemical Industry. Cu/Zn-SOD was diluted with distilled
water to obtain a 4 mg/mL solution, and the resulting solution was
stored at 4 °C. Caffeine was dissolved with distilled water to
prepare a 2 mmol/L solution. A 0.05 M Tris-HCl buffer solution with
pH = 8.2 was prepared. All other reagents are analytically pure.
from Doulai Biotechnology Co., Ltd. Caffeine (98% purity) was purchased
from Tokyo Chemical Industry. Cu/Zn-SOD was diluted with distilled
water to obtain a 4 mg/mL solution, and the resulting solution was
stored at 4 °C. Caffeine was dissolved with distilled water to
prepare a 2 mmol/L solution. A 0.05 M Tris-HCl buffer solution with
pH = 8.2 was prepared. All other reagents are analytically pure.
3
Trospium Chloride and Caffeine Bioavailability
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Trospium chloride and caffeine were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Sodium chloride (NaCl), sodium dihydrogen phosphate dihydrate (NaH2PO4 2H2O), 8N NaOH, taurocholic acid (TC), and oleic acid (OA) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Propantheline bromide was purchased from Funakoshi Co., Ltd (Tokyo, Japan). Ambenonium dichloride was purchased from Toronto Research Chemicals (Ontario, Canada). Egg yolk lecithin (EL) was purchased from NOF Corporation (Tokyo, Japan). Glyceryl mono-oleate (GM) was purchased from Nippon Surfactant Industries Co., Ltd (Tokyo, Japan). A cellulose dialysis membrane was purchased from As-One Corporation (Osaka, Japan).
The Food and Drug Adminstration (FDA) breakfast ingredients were purchased from the local market (bacon (Itoham Foods Inc, Japan), toast (Choujuku bread, Pasco Shikishima Corporation, Japan), egg (Odama mix, I.T.S Farm Co., Ltd, Japan), hash browns (Hoshino potato, Heinz Japan Ltd, Japan), whole milk (Oishii Megumilk Snow Brand Milk, MEGMILK SNOW BRAND Co., Ltd, Japan), and butter (Hokkaido-butter, MEGMILK SNOW BRAND Co., Ltd, Japan)).
The Food and Drug Adminstration (FDA) breakfast ingredients were purchased from the local market (bacon (Itoham Foods Inc, Japan), toast (Choujuku bread, Pasco Shikishima Corporation, Japan), egg (Odama mix, I.T.S Farm Co., Ltd, Japan), hash browns (Hoshino potato, Heinz Japan Ltd, Japan), whole milk (Oishii Megumilk Snow Brand Milk, MEGMILK SNOW BRAND Co., Ltd, Japan), and butter (Hokkaido-butter, MEGMILK SNOW BRAND Co., Ltd, Japan)).
4
Chemical Compound Procurement for Biological Assays
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SDS, sorbitol, DTT, NaCl, and LiCl were purchased from Wako Pure Chemical Industries (Osaka, Japan). Fluconazole, voriconazole, hygromycin B, caffeine, and monensin sodium salt were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Congo red was purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). CaCl2 was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Tunicamycin was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Brefeldin A (BFA) was purchased from Funakoshi Co., Ltd. (Tokyo, Japan). Human serum [(from male AB clotted whole blood), USA origin, sterile-filtered] (Product ID: H6914) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Antifungal and Stress Resistance Assay Protocol
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Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Cefotaxime sodium, D-glucose, agar, NaCl, KCl, sorbitol, H2O2, DTT, MMS, and AMPH-B were purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). Hygromycin B, caffeine, FLCZ, VRCZ, and N-phenylthiourea were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Congo Red and menadione were purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). Hipolypeptone was purchased from Nihon Pharmaceutical Co., Ltd. (Tokyo, Japan). Tunicamycin (TM) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Sodium dodecyl sulfate (SDS) was purchased from Nippon Gene Co., Ltd. (Tokyo, Japan). Tween 80 was purchased from MP Biomedicals LLC (Santa Ana, MO, USA).
6
Yerba Mate Antioxidant and Anti-Inflammatory Properties
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Rhizopus oligosporus (R. oligosporus) KCCM 11948P was obtained from Korean Culture Center of Microorganisms (KCCM, Seoul, Korea) (Park et al. 2017 (link)). RAW264.7 murine macrophage cells were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). Yerba mate was purchased from NATURE (Chungju-si, Korea). Ferulic acid, gallic acid (GA), chlorogenic acid, caffeic acid, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), Folin-Ciocalteu reagent, lipopolysaccharides (LPS), α-glucosidase from Saccharomyces cerevisiae, and dimethyl sulfoxide (DMSO) were bought from Sigma-Aldrich (St. Louis, MO, USA). Caffeine was obtained from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan) and EZ-Cytox was bought from DOGEN BIO (Seoul, Korea). Rutin, and 2,2′-azobis (2-methylpropionmidine) dihydrochloride (AAPH) were purchased from Acros Organics (Geel, Belgium). Fluorescein was bought from Alfa Aesar (Haverhill, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gene Depot (Barker, TX, USA), penicillin and streptomycin (PS) were obtained from Invitrogen (Carlsbad, CA, USA).
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