Fgf 2 quantikine elisa dfb50

All reagents were purchased from Sigma-Aldrich (Munich, Germany), including 4-styrenesulfonic acid sodium salt hydrate (NaSS) (cat. no. 434574), unless otherwise indicated. The anti-TGF-β (V) antibody was obtained from Invitrogen (ThermoFisher Scientific, Karlsruhe, Germany), and the anti-FGF-2 (C-18) antibody was obtained from Santa Cruz Biotechnology (Heidelberg, Germany). The biotinylated secondary antibodies and the ABC reagent were obtained from Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The AAVanced Concentration Reagent was purchased at System Bioscience (Heidelberg, Germany). The TGF-β Quantikine Enzyme-Linked Immunosorbent Assay (ELISA; DB100B) and the FGF-2 Quantikine ELISA (DFB50) were obtained from R&D Systems (Mannheim, Germany). The Cell Proliferation Reagent WST-1 was obtained from Roche Applied Science (Mannheim, Germany).

All reagents were from Sigma (Munich, Germany), unless otherwise identified. Recombinant TGF-β (rTGF-β) was purchased at Peprotech (Hamburg, Germany) and the dimethylmethylene blue dye at Serva (Heidelberg, Germany). The anti-FGF-2 (C-18) and anti-SOX9 (C-20) antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type-II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, USA), the anti-type-I collagen (AF-5610) antibody from Acris (Hiddenhausen, Germany), and the anti-type-X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and the ABC reagent were obtained from Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The FGF-2 Quantikine ELISA (DFB50) was from R&D Systems (Wiesbaden, Germany) and the type-II, −I, and -X collagen ELISAs from Antibodies-Online (Aachen, Germany).