Anti hemagglutinin ha

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-hemagglutinin (HA) is a laboratory reagent used to detect and measure the presence of the hemagglutinin protein, which is a surface glycoprotein found on the envelope of influenza viruses. This reagent can be used in various immunological assays to identify and quantify the hemagglutinin antigen.

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4 protocols using anti hemagglutinin ha

Immunoprecipitation and Western Blot Analysis

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MG132, cycloheximide, and 3-MA were obtained from Sigma-Aldrich (St. Louis, MO, United States); anti-Myc, anti-Flag, anti-hemagglutinin (HA), anti-GAPDH, protein G agarose used for IP and horseradish peroxidase-conjugated secondary antibodies were from Santa Cruz Biotechnology (Dallas, TX, United States); anti-RUNX2 were obtained from Cell Signaling Technology (Beverly, MA, United States); anti-TRIM16, anti-CHIP and anti-HSP70 were from Proteintech (Rosemont, IL, United States); anti-COL1A1 was from Servicebio (Wuhan, China).

Zhao Y., Zhai Q., Liu H., Xi X., Chen S, & Liu D. (2021). TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2. Frontiers in Cell and Developmental Biology, 8, 625105.

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IL-17A Signaling Pathway in Bronchial Epithelial Cells

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Normal human bronchial epithelial cells (BEAS-2B) were maintained in defined keratinocyte-SFM (Gibco by Life Technologies, USA) at 37°C under 5% CO₂. Recombinant human IL-17A (317-ILB-050), mouse monoclonal anti-human IL-17R-allophycocyanin (APC)-conjugated antibody, and an isotype control antibody were purchased from R&D Systems (USA). IL-17A was reconstituted to 100 μg/ml in 4 mM HCl. A rabbit polyclonal anti-IL-17R antibody, to detect the N-terminal extracellular domain of IL-17R, anti-hemagglutinin (HA) and goat polyclonal anti-GAPDH antibodies were obtained from Santa Cruz Biotechnology (USA). Rabbit poly-clonal anti-phospho-Akt (Ser473) (p-Akt), anti-phosphoglycogen synthase kinase-3β (Ser9) (p-GSK-3β), anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK), and rabbit monoclonal anti-phospho-p38 MAPK (Thr180/Tyr182) (p-p38) antibodies were obtained from Cell Signaling (USA). U0126 (a highly selective inhibitor of MEK1 and MEK2), LY294002 (an inhibitor of phosphatidylinositol 3-kinase, PI3K), and SB203580 (a selective inhibitor of p38 MAPK) were obtained from Cell Signaling.

Lee K.H., Lee C.H., Woo J., Jeong J., Jang A.H, & Yoo C.G. (2018). Cigarette Smoke Extract Enhances IL-17A-Induced IL-8 Production via Up-Regulation of IL-17R in Human Bronchial Epithelial Cells. Molecules and Cells, 41(4), 282-289.

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Antibody Validation for Protein Analysis

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Mouse monoclonal anti-hemagglutinin (HA) and rabbit polyclonals anti-RhoGDI, anti-HRAS, anti-panRAS, anti-PEA15, anti-RSK1 anti–p-ERK, and anti-ERK2 were from Santa Cruz Biotechnology, Santa Cruz, CA. Rabbit polyclonal anti-p-RSK-1, anti-ELK1, and anti-p-ELK1 were from Cell Signaling, Billerica, MA. Rabbit polyclonal anti-caveolin and anti–lamin A were from BD Biosciences, San Jose, CA. Mouse monoclonal anti–transferrin receptor was from Zymed Laboratories, Waltham, MA. Rabbit polyclonal anti-calreticulin was from Calbiochem.

Herrero A., Casar B., Colón-Bolea P., Agudo-Ibáñez L, & Crespo P. (2016). Defined spatiotemporal features of RAS-ERK signals dictate cell fate in MCF-7 mammary epithelial cells. Molecular Biology of the Cell, 27(12), 1958-1968.

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Antibody Characterization for Protein Analysis

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The following antibodies were purchased from Santa Cruz Biotechnology: anti–β-catenin (sc-7963), anti-CHIP (sc-66830), anti–c-Myc (sc-40), anti-GST (sc-138), anti–hemagglutinin (HA) (sc-7392), anti-MAPK3 (sc-94), anti-OTUB1 (sc-130458), anti-p53 (sc-126), anti-PGAM5 (sc-161156), anti–PP2B-A (sc-9070), anti-PRMT1 (sc-59648), anti-tubulin (sc-23948), anti-UB (sc-8017), anti-V5 (sc-271944), and goat anti-rabbit IgG (sc-2004). Goat anti-mouse IgG antibody (31438) was from Thermo Fisher Scientific. Penta-His antibody (34660) was from Qiagen. Anti-flag (M2) antibody (F3165) was from Sigma-Aldrich. p53 protein (SP-452-020) was from R&D Systems. Biotin-conjugated wt UB was from Boston Biochem. Polymerase chain reaction (PCR) primers were from Integrated DNA Technologies and are listed in table S5.

Bhuripanyo K., Wang Y., Liu X., Zhou L., Liu R., Duong D., Zhao B., Bi Y., Zhou H., Chen G., Seyfried N.T., Chazin W.J., Kiyokawa H, & Yin J. (2018). Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer. Science Advances, 4(1), e1701393.

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