12 week old Abcc5−/− mice and wild-type (wt) littermate controls were fasted overnight (16hrs). The mice were weighed and fasting glucose levels measured from whole blood via tail bleed under local anaesthesia (5% EMLA cream, Eutectic Mixture of Local Anesthetics, Lidocaine/Prilocaine, AstraZeneca, UK). An oral gavage of 20% glucose solution in 0.9% NaCl at 2g/kg of body mass was administered and whole blood glucose measurement taken at 15min, 30min, 60min and 120min after the gavage. The glucose measurements were performed by using a hand-held AlphaTRAK glucometer for pets (Abbott Laboratories).
Lidocaine prilocaine
Manufactured by AstraZeneca
Sourced in Australia
Lidocaine/Prilocaine is a topical anesthetic product. It contains the active ingredients lidocaine and prilocaine, which are used to provide local numbing or anesthesia to the skin and underlying tissues.
Automatically generated - may contain errors
4 protocols using lidocaine prilocaine
1
Glucose Tolerance Test in Abcc5-/- Mice
2
Glucose Homeostasis Assessment Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fed blood glucose was monitored regularly from small tail incisions after administration of a local anaesthetic (EMLA cream, Eutectic Mixture of Local Anesthetics, Lidocaine/Prilocaine, AstraZeneca), using Lithium-Heparin microvette tubes (Sarstedt). Plasma glucose was measured using an Analox Glucose Analyser GM9.
IPGTTs were conducted after an overnight fast. Blood glucose was measured by tail incision immediately before, and then 30, 60 and 120 minutes after injection with 2 g of glucose per kg body weight (20% glucose in 0.9% NaCl). An additional time point (15 minutes) was added for the IPGTT at 13 weeks of age.
IPITTs were conducted after a 4h fast. Blood glucose was measured by tail incision immediately before and 15, 30, 45, 60 and 90 minutes after intraperitoneal injection of insulin (0.75 U/kg
BW).
IPGTTs were conducted after an overnight fast. Blood glucose was measured by tail incision immediately before, and then 30, 60 and 120 minutes after injection with 2 g of glucose per kg body weight (20% glucose in 0.9% NaCl). An additional time point (15 minutes) was added for the IPGTT at 13 weeks of age.
IPITTs were conducted after a 4h fast. Blood glucose was measured by tail incision immediately before and 15, 30, 45, 60 and 90 minutes after intraperitoneal injection of insulin (0.75 U/kg
BW).
3
Intramuscular Genioglossal EMG Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genioglossal activity was recorded from four monopolar intramuscular wire electrodes inserted percutaneously to a depth of 25 mm, at locations 10 and 20 mm posterior to the posteroinferior margin of the mandible and 5 mm lateral to the midline (each side). Each wire was inserted using a 25-gauge needle, 20-30 minutes after the application of topical local anaesthetic (Lidocaine—Prilocaine; AstraZeneca Pty Ltd, NSW, Australia) to the submental area. GG EMG signals were amplified and band-pass filtered from 30 to 3 kHz (model P511, Grass TeleFactor; Grass Technologies, West Warwick, RI).
4
Intramuscular EMG Electrode Placement for Genioglossus Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GG activity was recorded using four monopolar intramuscular wire electrodes referenced to a surface electrode on the left cheek and grounded by a flexible strap on the left shoulder. Electrodes were stainless steel Teflon coated 50 µm wire electrodes (A-M Systems Inc, Sequim Washington) of which 0.5 mm of the tip was bared. Each wire was inserted percutaneously via a 25-gauge hypodermic needle after numbing of the submental area with 20-30 min of surface anesthesia (Lidocaine-Prilocaine; AstraZeneca Pty Ltd, NSW, Australia). The GG wires were inserted between 10 and 20 mm from the posteroinferior margin of the mandible, at a depth of 2-2.5 cm from the surface and ~5mm each side of the midline. The range of wire placements into the muscle was performed to maximize chances of identifying unique SMUs and to ensure sufficient EP and ET units were recorded as these are typically found more superficially [27] . GG EMG signals were band-pass filtered from 30 to 10 kHz (model P511, Grass TeleFactor; Grass Technologies, West Warwick, RI).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!