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Cc 3516

Manufactured by PromoCell
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The CC-3516 is a laboratory device designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The product specifications and technical details are available upon request.

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Lab products found in correlation

Pump system, by Ibidi (2 mentions) Type 1 collagen, by BD (2 mentions) C 12271, by PromoCell (2 mentions) Cc 4176, by PromoCell (2 mentions) C 22011, by PromoCell (2 mentions) Slide 1 0.4 luer, by Ibidi (1 mentions) μ slide i0.4 luer, by Ibidi (1 mentions)

2 protocols using cc 3516

1

Shear Stress Conditioning of Aortic Endothelial Cells

Cited 1 time
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Human aortic endothelial cells (HAECs) were obtained commercially (GIBCO, C0065C or PromoCell, C-12271) and cultured according to the manufacturer’s standard protocol. HAECs were seeded at various density to meet the purposes of different studies, and maintained in EC medium containing growth supplements (EBM-2, Lonza, CC-3516, CC-4176 or PromoCell, C-22011) with 2% FBS. HAECs between passage 6 and 7 were used for experiments. For shear experiments, confluent HAECs in 100 mm dishes were exposed to steady laminar shear (LS, 15 dyn/cm2), or oscillatory shear (OS,  ±5 dyn/cm2) conditions for 2 days using a cone-and-plate shear device as previously described [20 (link), 21 (link)]. For shear experiments with gain of function or loss of function components, the commercially available Ibidi pump system (Ibidi, Germany) was used to reduce reagent use, and set up according to manufacturer instructions. LS (15 dyn/cm2) or OS (±5 dyn/cm2) was applied for 2 days to 80% confluent HAECs cultured overnight on microchannel slides (µ-Slide I0.4 Luer, Ibidi, Germany) coated with 40 μg/ml collagen (Collagen Type I, BD Biosciences 35–4236).
Wang Y., Sun H.Y., Kumar S., Puerta M.D., Jo H, & Rezvan A. (2018). ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins. Laboratory Investigation; a Journal of Technical Methods and Pathology, 99(3), 305-318.
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2

Human Aortic Endothelial Cell Shear Experiments

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human aortic endothelial cells (HAECs) were obtained commercially (GIBCO, C0065C or PromoCell, C-12271) and cultured according to the manufacturer’s standard protocol. HAECs were seeded at various density to meet the purposes of different studies, and maintained in endothelial cell medium containing growth supplements (EBM-2, Lonza, CC-3516, CC-4176 or PromoCell, C-22011) with 2% FBS. HAECs between passage 6 and 7 were used for experiments. For shear experiments, confluent HAECs in 100 mm dishes were exposed to steady laminar shear (LS, 15dyn/cm2), or oscillatory shear (OS, ±5dyn/cm2) conditions for 2 days using a cone-and-plate shear device as previously described.20 (link), 21 (link) For shear experiments with gain of function or loss of function components, the commercially available Ibidi pump system (Ibidi, Germany) was used to reduce reagent use, and set up according to manufacturer instructions. Steady laminar shear (LS, 15dyn/cm2) or oscillatory shear (OS, ±5dyn/cm2) was applied to 80% confluent HAECs cultured overnight on microchannel slides (μ-Slide I0.4 Luer, Ibidi, Germany) coated with 40μg/ml collagen (Collagen Type I, BD Biosciences 35-4236).
Wang Y., Sun H.Y., Kumar S., Puerta M.D., Jo H, & Rezvan A. (2018). ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins. Laboratory Investigation; a Journal of Technical Methods and Pathology, 99(3), 305-318.
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