Xgen methyl seq dna library prep kit

Manufactured by Integrated DNA Technologies

Sourced in United States

The XGen™ Methyl-Seq DNA Library Prep Kit is a product from Integrated DNA Technologies designed for the preparation of DNA libraries for methylation sequencing. The kit provides the necessary reagents and protocol for the construction of sequencing-ready libraries from DNA samples.

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Lab products found in correlation

Ez dna methylation gold kit, by Zymo Research (2 mentions) Quantiflour dsdna system, by Promega (2 mentions)

2 protocols using xgen methyl seq dna library prep kit

Cell-free DNA Bisulfite Sequencing

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Bisulfite conversion and purification of cell-free DNA samples were conducted using EZ DNA Methylation-Gold Kit (Zymo research, D5006, USA), following the manufacturer’s instructions. Subsequently, bisulfite-converted DNA samples were denatured to single stranded then processed with Adaptase™ technology adding adapters and unique dual indexes using xGen™ Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies, 10009824, USA). DNA concentrations were measured using the QuantiFlour dsDNA system (Promega, USA).

Pham T.M., Phan T.H., Jasmine T.X., Tran T.T., Huynh L.A., Vo T.L., Nai T.H., Tran T.T., Truong M.H., Tran N.C., Nguyen V.T., Nguyen T.H., Nguyen T.H., Le N.D., Nguyen T.D., Nguyen D.S., Truong D.K., Do T.T., Phan M.D., Giang H., Nguyen H.N, & Tran L.S. (2023). Multimodal analysis of genome-wide methylation, copy number aberrations, and end motif signatures enhances detection of early-stage breast cancer. Frontiers in Oncology, 13, 1127086.

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Bisulfite-based DNA Methylation Analysis

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According to the manufacturer’s instructions, bisulfite conversion and cfDNA purification were prepared by EZ DNA Methylation-Gold Kit (Zymo Research, D5006, USA). DNA library was prepared from bisulfite-converted DNA samples using xGen Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies, 10009824, USA) with Adaptase technology, according to the manufacturer’s instructions. The QuantiFlour dsDNA system (Promega, USA) was used to analyze the concentration of DNA.

Nguyen V.T., Nguyen T.H., Doan N.N., Pham T.M., Nguyen G.T., Nguyen T.D., Tran T.T., Vo D.L., Phan T.H., Jasmine T.X., Nguyen V.C., Nguyen H.T., Nguyen T.V., Nguyen T.H., Huynh L.A., Tran T.H., Dang Q.T., Doan T.N., Tran A.M., Nguyen V.H., Nguyen V.T., Ho L.M., Tran Q.D., Pham T.T., Ho T.D., Nguyen B.T., Nguyen T.N., Nguyen T.D., Phu D.T., Phan B.H., Vo T.L., Nai T.H., Tran T.T., Truong M.H., Tran N.C., Le T.K., Tran T.H., Duong M.L., Bach H.P., Kim V.V., Pham T.A., Tran D.H., Le T.N., Pham T.V., Le M.T., Vo D.H., Tran T.M., Nguyen M.N., Van T.T., Nguyen A.N., Tran T.T., Tran V.U., Le M.P., Do T.T., Phan T.V., Nguyen H.D., Nguyen D.S., Cao V.T., Do T.T., Truong D.K., Tang H.S., Giang H., Nguyen H.N., Phan M.D, & Tran L.S. (2023). Multimodal analysis of methylomics and fragmentomics in plasma cell-free DNA for multi-cancer early detection and localization. eLife, 12, RP89083.

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