C57bl 6j mouse

Manufactured by Charles River Laboratories

Sourced in Japan

The C57BL/6J mouse is a widely used inbred strain of laboratory mouse. It is a common model organism in biomedical research, due to its well-characterized genome and physiological traits.

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Lab products found in correlation

Cm4000, by Thermo Fisher Scientific (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Hepes, by Solarbio (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Collagenase type 4, by Merck Group (1 mentions) Percoll, by Cytiva (1 mentions)

Close spelling variants of this product

C57BL/6J mouse embryos C57Bl/6J mouse strain C57BL/6J

4 protocols using c57bl 6j mouse

Anti-GPC3 Antibody Inhibits Tumor Growth in Syngenic Mouse Model

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Example 2

Anti-tumor activity of anti-GPC3 antibody in syngenic mouse model using Hepa1-6 cell line expressing human GPC3

Hepa1-6/hGPC3 cells were cultured using cell culture flasks in an incubator (set at 37° C. and 5% CO₂). The cells were detached from the flasks with trypsin and washed with D MEM containing 10% (v/v) FBS, 0.6 mg/mL G418. Then the cells were re-suspended in D-MEM (2×10⁸cells/mL), and an equal volume of Matrigel was added. The cell concentrations for implantation were 1×10⁸cells/mL. The cells were inoculated subcutaneously into the right flank of each C57BL/6J mouse (Charles River Laboratories Japan) (1×10⁷cells/mouse). Once palpable tumors were established, animals were randomized into testing groups so that each group had similar mean tumor volumes when the study started. Either 1 or 5 mg/kg of mouse GC33 anti-human GPC3 monoclonal antibody [WO2006/006693] diluted in PBS, or PBS as a vehicle control was injected at day 14, 21 and 28 intravenously after tumor inoculation. Mouse GC33 showed inhibition of tumor growth with dose dependency compared to vehicle control (FIG. 1).

US11767362B1. Methods of treating cancers using PD-1 axis binding antagonists and anti-GPC3 antibodies (2023-09-26). Chugai Seiyaku Kabushiki Kaisha [JP], Genentech, Inc. [US]. Inventors: Mika Endo [JP], Atsuhiko Kato [JP], Toshihiko Ohtomo [JP], Kenji Adachi [JP], Yasuko Kinoshita [JP], Yoshinori Narita [JP].

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Isolation and Culture of Mouse Hepatocytes

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Primary hepatocytes were isolated C57BL/6J mouse (4-week-old or 1-year-old, Charles River Japan) and prepared using the two-step collagenase perfusion method [15 (link)]. The primary culture of mouse hepatocyte from C57BL/6J was maintained with CM4000 (Thermo Fisher) changing every two days. The medium was contained with 0.1 μM dexamethasone, 6.25 μg/mL insulin, 6.25 μg/mL transferrin, 6.25 ng/mL selenous acid, 1,25 ng/mL BSA, 5.35 μg/mL linoleic acid, 2mM GlutaMAX and 15 mM HEPES 7.4 [13 (link)].

Cho J., Tsugwa Y., Imai Y, & Imai T. (2021). Chorionic gonadotropin stimulates maternal hepatocyte proliferation during pregnancy. Biochemical and biophysical research communications, 579, 110-115.

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Chronic Social Defeat Stress Model

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Adult male and female C57BL/6 J mice (Jackson Laboratories, Bar Harbor, ME) were randomly assigned to control or CNSDS conditions, and then completed Control or CNSDS protocols as previously described^{27 (link),28}. In the control condition, a male and female C57BL/6 J mouse interacted for 5 min daily, followed by co-housing with a perforated Plexiglas cage divider. In the CNSDS condition, a male and female C57BL/6 J mouse were placed in the home cage of a retired breeder CD-1 male (Charles River Laboratory, Wilmington, MA) for 10 consecutive daily 5-minute defeat sessions. After each defeat session, the male C57BL/6 J mouse was co-housed with a novel CD-1 male, separated by a cage divider. The female was co-housed with the aggressor CD-1, again separated by a cage divider but allowing sensory interaction, but no physical contact. Co-housing alternated each day. Thus, the male and female pair were each housed with the attacking CD-1 on half of CNSDS days. Following CNSDS, mice were pair-housed in standard clear Plexiglas mouse cages with corncob bedding with perforated Plexiglas separating the pair of mice in the cage. Following CNSDS, all mice were food-deprived and maintained at 90% of their free-feeding body weight and fed daily with standard lab chow at least 1-hour after behavior testing.

Dieterich A., Liu T, & Samuels B.A. (2021). Chronic non-discriminatory social defeat stress reduces effort-related motivated behaviors in male and female mice. Translational Psychiatry, 11, 125.

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Mouse Hepatocyte Isolation Protocol

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All animal experiment procedures were conducted in compliance with the approval of the Animal Ethics Committee at ShanghaiTech University (20200713002). Mouse hepatocytes were isolated from 8-week-old male C57BL/6 J mouse (Charles River Laboratories) using the procedure of standard two-steps collagenase perfusion method^{46 (link)}. In brief, the liver was pre-perfused through the portal vein with perfusion solution (1 × EBSS without Ca²⁺ and Mg²⁺(Sangon Biotech) supplemented with 0.5 mM EGTA (Sigma-Aldrich)) for 3–5 min and then perfused with collagenase solution (0.2 mg/ml collagenase type IV (Sigma-Aldrich), 10 mM HEPES (Solarbio), 1× EBSS with Ca²⁺ and Mg²⁺ (Sangon Biotech)) at 2–3 ml/min. The extracted liver was mechanically digested with sterile scissors and then filtered through a 70 μm filter membrane. The cells were collected via centrifugation at 60 g for 2 min. Then the cells were resuspended using 40% Percoll (Cytiva) diluted with DMEM supplemented with 10% FBS and centrifuged at 1000 rpm for 5 min to remove dead and non-hepatic cells. Purified hepatocytes were then counted and used for further experiments.

Yuan Y., Wang C., Zhuang X., Lin S., Luo M., Deng W., Zhou J., Liu L., Mao L., Peng W., Chen J., Wang Q., Shu Y., Xue Y, & Huang P. (2022). PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest. Nature Communications, 13, 5237.

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