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Chromasolv lc ms water

Manufactured by Honeywell
Sourced in United States

CHROMASOLV™ LC-MS water is a high-purity solvent designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is a purified, deionized, and filtered water that meets the stringent requirements for LC-MS analysis.

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2 protocols using chromasolv lc ms water

1

Mass Spectrometry Sample Preparation

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Dithiothreitol (DTT), iodoacetamide (IAA), Pierce formic acid (LC-MS grade), and Pierce HeLa protein digest standard were purchased from Thermo Fisher Scientific. CHROMASOLV™ LC-MS water and acetonitrile were products of Honeywell (Charlotte, NC, USA), MS-grade trypsin and Lys-C were from Promega (Madison, WI, USA). All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted.
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2

Protein Extraction and Digestion Protocol

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Mayer’s Hematoxylin solution (MHS16–500ml) and eosin Y solution (110116–500ml) were purchased from Sigma-Aldrich (St. Louis, MO). Scott’s bluing reagent (Cat. No. 6697–32) was purchased from Ricca Chemical Company (Arlington, TX). Ethanol was purchased from Decon laboratories Inc. USA. Pierce HeLa Protein Digest Standard, Pierce Formic Acid (FA) (LC-MS grade), Dithiothreitol (DTT), and iodoacetamide (IAA) were purchased from ThermoFisher Scientific (Waltham, MA). CHROMASOLV LC-MS water and acetonitrile were products of Honeywell (Charlotte, NC), MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted.
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