Microbca protein quantification kit

The ExoQuick ULTRA exosome isolation kit was purchased from SBI System Bioscience (Palo Alto, CA, USA). Anti-L1CAM biotinylated antibody was purchased from R&D Systems (Minneapolis, MN, USA). The human CD81 (Cluster of Differentiation 81) antigen ELISA kit was purchased from CUSABIO (Wuhan, China). ELISA kits for C7 (Complement component 7), FERMT3 (Fermitin Family Member 3), CAP1 (Adenylyl cyclase-associated protein 1), ENO1 (Enolase 1), and ZYX (Zyxin) were purchased from CLOUD-CLONE (Wuhan, China). Albumin/IgG removal kits were purchased from Merk (Shanghai, China). TMT 6-plex labeling kit, MicroBCA protein quantification kit, Streptavidin Plus UltraLink™ Resin, M-PER Mammalian Protein Extraction Reagent, 1M TEAB, and 50% Hydroxylamine were purchased from Thermo Scientific (Rockford, IL, USA). Sequence grade trypsin was purchased from Promega (Madison, WI, USA). Dithiothreitol and indole-3-acetic acid were purchased from GE Healthcare (Shanghai, China).

Primary antibodies used in the Western blot studies are as follows: anti-human Grp75, CD9, OCT4 (CST, Danvers, USA), anti-human ALDH, CD44, CD133 (GeneTex, Irvine, USA), anti-human sytenin1 (Abcam, Cambridge, UK), anti-human CD64 (Proteintech, Wuhan, China), and anti-human GAPDH [Beyotime Institute of Biotechnology (Nanjing, China)]. HRP conjugated secondary antibodies are from Beyotime Institute of Biotechnology (Nanjing, China). Growth factors bFGF and EGF were purchased from Gibco. MicroBCA protein quantification kit was purchased from Thermo Scientific (USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan).