Human lung cancer A459 cells, retinal pigment epithelial RPE1 cells and embryonic kidney 293T cells were all from ATCC. A549 cells were cultured in F12K Nutrient Mixture (#21127-022 Gibco) supplemented with 10% FBS and 1% P/S. RPE1 and 293T cells were cultured in DMEM (10-014-CV, CORNING) supplemented with 10% FBS and 1% P/S. They were all maintained at 37 °C under 5% CO2 in a humidified incubator (Thermo, USA).
Dmem 10 014 cv
Manufactured by Corning
Sourced in United States
DMEM (10-014-CV) is a powdered cell culture medium formulated for the maintenance and growth of a variety of mammalian cell lines. It provides essential nutrients and supports cell proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Humidified incubator, by Thermo Fisher Scientific (1 mentions)
F 12k nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Fss500, by ExCell Bio (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Nb100 2331, by Novus Biologicals (1 mentions)
A2668, by Merck Group (1 mentions)
Goat anti rabbit igg secondary antibody a9169, by Merck Group (1 mentions)
Full range rainbow marker rpn800e, by GE Healthcare (1 mentions)
3 protocols using dmem 10 014 cv
1
Cell Culture of Human Cancer, Retinal, and Kidney Lines
2
Examine BK Channel Inhibition in HUASMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human umbilical artery smooth muscle cells (HUASMCs) were purchased from Shanghai Xinyu Biotechnology Company (Shanghai, China). HUASMCs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; 10-014-cv; Corning, New York, USA) containing 10% fetal bovine serum (FBS; FSS500; Excell Bio, Shanghai, China) at 37°C in an incubator with 95% air and 5% CO
2 after they reached 80% confluence. To examine the effect of BK channels on HUASMCs, the medium was supplemented with the 1, 5, and 10 μM BK channel inhibitor paxilline (ab141840; Abcam, Cambridge, UK). After 24‒72 h of treatment, the cells were collected for subsequent studies.
2 after they reached 80% confluence. To examine the effect of BK channels on HUASMCs, the medium was supplemented with the 1, 5, and 10 μM BK channel inhibitor paxilline (ab141840; Abcam, Cambridge, UK). After 24‒72 h of treatment, the cells were collected for subsequent studies.
3
Mesenchymal Stem Cell Culture Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DMEM (10-014-CV) and α-MEM (15-012-CV) for MSC cultures were obtained from Corning/Mediatech (Manassass, VA, USA). In glucose experiments, phenol red-free DMEM (A14430-01) was obtained from Gibco/Thermo Fisher and α-MEM for primary cells (17-305-CV) was obtained from Corning. For cell culture preparations, fetal bovine serum (FBS) was obtained from Atlanta Biologicals (S11550H; Flowery Branch, GA, USA) for primary MSC cultures and Gemini Bio-Products (100-106; Sacramento, CA, USA) for immortalized MSC cultures. For the propidium iodide (PI) uptake experiments, propidium iodide from Thermo Fisher (P3566) was diluted in DMEM at 5.0 μg/mL. For immunoblotting, rabbit polyclonal LC3 antibody (NB100-2331) was obtained from Novus Biologicals (Littleton, CO, USA) and goat anti-rabbit IgG secondary antibody (A9169) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Housekeeping gene anti-actin produced in rabbit (A2668) was obtained from Sigma-Aldrich. Protein ladder for all immunoblots was a Full Range Rainbow marker (RPN800E) from GE Life Sciences (Pittsburgh, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!