P pi3k antibody

Fluoxetine hydrochloride capsule (Flu) was purchased from Patheon France and was packaged by Lilly Suzhou Pharmaceutical Co., Ltd. Ginsenosides Rg1 (GRg1, B21057), and saikosaponin d (Ssd, B20150) were purchased from Yuanye Biotech (Shanghai, China). Lipopolysaccharide (LPS: L3129) was purchased from Sigma–Aldrich. A small-molecule-specific inhibitor of TLR4 signaling (TAK242, A3850) and PI3K/AKT inhibitor (LY294002, A8250) were from Apex Bio (Houston, Texas). TNF-α, IL-6, and IL-1β ELISA kits were purchased from BioProducts MD (Middletown, MD, USA). The antibodies to PI3K (ab178846), p-AKT (ab192623), AKT (ab179463), p-PTEN (ab76431), PTEN (ab170941), p-FOXO1 (ab131339), and FOXO1 (ab52857) were from Abcam, TLR4 antibody (AF7017) was from Affinity Biosciences, the p-PI3K-antibody (#4228) was from Cell signaling, the anti-β-actin (sc-47778) and goat anti-rabbit IgG (sc-516102) were from Santa Cruz Biotechnology.

RHu PDGF-BB, IL-1β and imatinib mesylate were purchased from MedChemExpress, United States (cat. nos. HY-P7055; HY-P7097; HY-50946); PDGF-BB polyclonal antibody, collagen II monoclonal antibody and aggrecan neo polyclonal antibody were purchased from Thermo Fisher Scientific, United Kingdom (cat. nos. PA5-88272; MA5-12789; PA1-1746); MMP3 antibody, MMP9 antibody, Adamts4 antibody, Adamts5 antibody and Caspase1 antibody were purchased from Abcam, United Kingdom (cat. nos. ab52915; ab76003; ab185722; ab41037; ab179515); PDGFR antibody, P-PDGFR antibody, P-P38 antibody and P-JNK antibody were purchased from Hangzhou Huaan Biotechnology Co., Ltd., China (cat. nos. SN0646; R1510-44; ER2001-52; ET1601-28); P-PI3K antibody, PI3K antibody, P-AKT antibody, AKT antibody, P38 antibody, JNK antibody, P-ERK antibody, ERK antibody, β-actin antibody and NLRP3 antibody were purchased from Cell Signaling Technology, United States (cat. nos.17366; 4292; 9271; 4691; 9212S; 9252S; 4370; 4695; 4970; ab263899); IL-1β antibody was purchased from ABclonal Technology Co., Ltd., China (cat. no. A1112).

Icariin (United States, 489-32-7) and ferric ammonium citrate (FAC, United States, 1185-57-5) were purchased from Sigma-Aldrich (Supplementary Figure S1). Cleaved caspase-3 (Asp175) antibody (United States, #9661), MFN2 antibody (United States, #11925), DRP1 antibody (United States, #8570), COX IV antibody (United States, #4850), RUNX-2 antibody (United States, #12556), active β-catenin antibody (United States, #19807), cyclin D1 antibody (United States, #2922), PI3K antibody (United States, #4257), P-PI3K antibody (United States, #4228), AkT antibody (United States, #4691), P-AkT antibody (United States, #4060), mTOR antibody (United States, #2972), P-mTOR antibody (United States, #5536), P-ERK antibody (United States, #4370), ERK antibody (United States, #4695), P-p38 antibody (United States, #4511), p38 antibody (United States, #8690), P-JNK antibody (United States, #4668), JNK antibody (United States, #9252) were all purchased from Cell Signaling Technology. BAX antibody (United States, 50599-2-Ig), FIS1 antibody (United States, 10956-1-AP), cytochrome C antibody (United States, 66264-1-Ig) and osteopontin (OPN) antibody (United States, 22952-1-AP) were all purchased from Proteintech (United States, 50599-2-Ig). Bcl-2 antibody was purchased from R&D system (United States, MAB8272). Anti-beta actin antibody was purchased from BOSTER (China, BM3873).