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3 protocols using cyclohexanone

1

Comparative Analysis of Edible Oil Oxidation

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The three oils used in this study were coconut oil (Organic extra virgin coconut oil from PLANTIS®, Barcelona, Spain), rapeseed oil (Organic cold pressed rapeseed oil Terpenic lab S.L., Barcelona, Spain) and grape seed oil (Refined grape seed Terpenic lab S.L., Barcelona, Spain). Three different batches of each type of oil were purchased in a local market.
2-thiobarbituric acid, and fatty acid methyl esters were obtained from Sigma-Aldrich Chemical (Steinheim, Germany). Boron/methanol trifluoride and heptane were purchased from Merck (Whitehouse Station, NJ, USA). Potassium hydroxide, hexane, cyclohexanone, hydrochloric acid, trichloroacetic acid and ammonium sulfate were from Panreac (Barcelona, Spain).
Salmonella typhimurium strains TA97a and TA98 and the Mutazime S9 mix at 10% from livers of Aroclor 1254-induced rats were purchased from Moltox (Boone, NC, USA). Nutrient broth and Phosphate Buffered Saline (PBS) tablets were obtained from Oxoid (Basingtone, UK). 4-nitro-o-phenylenediamine (NPD), 2-aminoanthracene (AA) and 2-aminofluorene (AF), histidine and biotin were obtained from Sigma-Aldrich.
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2

Meat and Fat Composition Analysis

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Fresh beef and pork meat (loin) and pork back fat were obtained from a local meat market. Sunflower oil (Urzante S.L, Navarra, Spain) was obtained in a local market.
Carrageenan (kappa-carrageenan) was kindly donated by Cargill (San Sebastián, Spain).
Cholesterol, 5α-cholestane, thiobarbituric acid, fatty acid methyl esters and Polysorbate 80 were purchased from Sigma-Aldrich Chemical (Steinheim, Germany). 19hydroxycholesterol was obtained from Steraloids (Wilton, NH, USA). Tri-sil® reagent was obtained from Pierce (Rockford, IL, USA). Acetone, chloroform, ethyl acetate, methanol, hexane, 2-propanol, hydrochloric acid, cyclohexanone, trichloroacetic acid, potassium chloride, potassium hydroxide, anhydrous sodium sulphate and sodium phosphate were obtained from Panreac (Barcelona, Spain). hexane for gas chromatography, dichloromethane for gas chromatography and boron trifluoride/methanol were from Merck (Whitehouse Station, NJ, USA). Strata NH 2 (55 µm, 70 A) 500 mg/3 mL Solid Phase Extraction cartridges were obtained from Phenomenex (Torrance, CA, USA).
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3

Comprehensive Lipid Extraction and Analysis

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Cholesterol, stigmasterol, 5α-cholestane, heptadecanoic acid, α-tocopherol, tocopherol acetate, ammonium thyocianate and thiobarbituric acid were purchased from Sigma-Aldrich Chemical (Steinheim, Germany). 19-hydroxyCholesterol was obtained from Steraloids (Wilton, NH, USA). Tri-sil ® reagent was obtained from Thermo-Scientific (Rockford, IL, USA). Hexane, heptane, acetone, chloroform, ethyl acetate, butanol, methanol, 2-propanol, hydrochloric acid, cyclohexanone, trichloroacetic acid, potassium chloride, potassium hydroxide, ammonium iron (II) sulphate, barium chloride, anhydrous sodium sulphate and sodium phosphate were obtained from Panreac (Barcelona, Spain). Strata NH 2 (55 µm, 70 A) 500 mg / 3 mL Solid Phase Extraction cartridges were obtained from Phenomenex (Torrance, USA). Sunflower oil was purchased in a major local distributor.
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