Lox stop lox rosa diphtheria toxin mice

Experiments were performed on male fat‐free (FF) and wild‐type (WT) littermate control mice on a C57BL/6J background. FF mice were generated by crossing adiponectin‐Cre mice (The Jackson Laboratory, Bar Harbor, ME, USA: 028020) with lox‐stop‐lox‐Rosa diphtheria toxin mice (The Jackson Laboratory: 010527) (Collins et al., 2020 ; Wu et al., 2018 ). The resulting FF mice constitutively have a complete absence of adipose tissue from birth. These mice and DTA/+ littermate WT control were maintained at thermoneutrality (30°C) on a chow diet (10% fat) with free cage activity. Exact animal numbers used in each experiments are listed in the figure legends. Complement factor D constitutive knockout mice were used to determine the role of adipsin in the FF muscle physiology phenotype (a generous gift from Drs Atkinson and Wu; Wu et al., 2018 ).