Anti trinitrophenyl ige antibody clone ige 3

The following materials were commercially obtained from the sources indicated: ATP, an anti-dinitrophenyl IgE antibody (clone SPE-7), p-nitrophenyl-β-d-2-acetoamide-2-deoxyglucopyranoside, probenecid and N-succinyl-Ala-Ala-Pro-Phe-pNA from Sigma-Aldrich (St. Louis, MO, USA), fetal bovine serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), an anti-trinitrophenyl IgE antibody (clone IgE-3), an FITC-conjugated rat anti-mouse IgE antibody, a phycoerythrin-conjugated rat anti-mouse CD117 antibody and Fc blocker (clone 2.4G2) from BD Biosciences (San Diego, CA, USA), trinitrophenyl bovine serum albumin (TNP-BSA) from LSL (Tokyo, Japan), H-D-Ile-Pro-Arg-pNA (S-2288) from Chromogenix (Milano, Italy), N-(4-methoxyphenylazoformyl)-Phe-OH potassium salt (M-2245) from Bachem AG (Bubendorf, Switzerland), thapsigargin from Merck Millipore (Billerica, MA, USA), Fura-2/AM from Dojindo Laboratories (Kumamoto, Japan), recombinant mouse IL-9 from PeproTech (Rocky Hill, NJ, USA) and recombinant mouse IL-3 from R & D Systems (Minneapolis, MN, USA). Murine SCF was prepared by baculovirus expression system with Sf9 cells according to the procedure described previously [16 (link)]. All other chemicals were commercial products of reagent grade.

The following materials were commercially obtained from the sources indicated: Dexamethasone, p-nitrophenyl-β-d-2-acetoamide-2-deoxyglucopyranoside, compound 48/80, an anti-dinitrophenyl IgE antibody (clone SPE-7), N-succinyl-Ala-Ala-Pro-Phe-pNA, mitomycin C, substance P, and dinitrophenyl human serum albumin (DNP-HSA) from Sigma-Aldrich (St. Louis, MO, USA), Toluidine blue, Safranin-O, and Evans blue from Wako Pure Chemical Industries (Osaka, Japan), an anti-trinitrophenyl IgE antibody (clone IgE-3) from BD Biosciences (San Diego, CA, USA), trinitrophenyl bovine serum albumin (TNP-BSA) from LSL (Tokyo, Japan), H-d-Ile-Pro-Arg-pNA (S-2288) from Chromogenix (Milano, Italy), N-(4-Methoxyphenylazoformyl)-Phe-OH potassium salt (M-2245) from Bachem AG (Bubendorf, Switzerland), an anti-G_αi2 antibody from Santa Cruz Biotechnology (Dallas, TX, USA), an anti-pan-actin antibody (clone C4), an anti-G_αi1 antibody, an anti-G_αi3 antibody, and thapsigargin from Merck Millipore (Billerica, MA, USA), a horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody and a HRP-conjugated anti-rabbit IgG antibody from Agilent Technologies (Santa Clara, CA, USA) and recombinant mouse IL-3 from R & D Systems (Minneapolis, MN, USA). All other chemicals were commercial products of reagent grade.