Uas denmark

Manufactured by Bloomington Drosophila Stock Center

The UAS-DenMark is a genetic tool used to visualize and study the dynamics of dendritic structures in Drosophila. It is a transgenic construct that expresses the fluorescent protein DenMark under the control of the upstream activating sequence (UAS) promoter.

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4 protocols using uas denmark

Genetic Toolkit for Drosophila Neuroscience

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All flies were maintained in standard cornmeal–yeast–agar medium at 25 °C. w[1118] (BL5905; wild-type control), Sh[mns] (BL24149), Sh[TKO] (BL76968), sss[P1] (BL16588), sss[MIC] (BL34309), Gad1[L352F] (BL6295), VGAT[MB01219] (BL23022), TrpA1[1] (BL26504), UAS-Sh RNAi #1 (BL53347), UAS-Rdl RNAi (BL52903), ELAV-Gal4 (BL458), ELAV-Gal4; UAS-Cas9 (BL67073), VGAT-Gal4 (II) (BL58980), VGAT-Gal4 (III) (BL58409), 23E10-Gal4 (BL49032), TH-Gal4 (BL8848), TDC2-Gal4 (BL9313), ChAT-Gal4 (BL6798), 121y-Gal4 (BL30815), 30y-Gal4 (BL30818), GAD1-LexA (BL60324), UAS-mLexA-VP16-NFAT (BL66542; CaLexA), UAS-DenMark, UAS-syt.eGFP (BL33065), lexAop-nSyb-spGFP1-10, UAS-CD4-spGFP11 (BL64315; syb:GRASP), UAS-SuperClomeleon (BL59847), and UAS-Epac1-camps (BL25407) were obtained from Bloomington Drosophila Stock Center. Hk[1] (101-119) was obtained from Kyoto Stock Center. UAS-Sh RNAi #2 (v104474) was obtained from Vienna Drosophila Resource Center. GAD1-Gal80, TH-LexA, and UAS-sytGCaMP6s; UAS-P2X₂ were gifts from Y. Li (Chinese Academy of Sciences), H. Tanimoto (Tohoku University), and R. Allada (Northwestern University), respectively.

Kim J.H., Ki Y., Lee H., Hur M.S., Baik B., Hur J.H., Nam D, & Lim C. (2020). The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission. Communications Biology, 3, 174.

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Diverse Drosophila Molecular Genetic Tools

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OR-mCD8::GFP [9 (link)], OR-Syt::GFP, OR-GAL4 [11 (link)], IR-GAL4 [52 (link)], GR-GAL4 [53 (link)] lines were from Leslie Vosshall, Barry Dickson, Richard Benton and John Carlson, respectively. Dpr-GAL4 and DIP-GAL4 [35 (link),37 (link)] lines were from Larry Zipursky. UAS-mCD8::GFP, UAS>STOP>mCD8::GFP (BL# 30125 and 30032) [54 (link)], UAS-Syt::GFP, ey-FLP, MZ19-mCD8::GFP [22 (link)], UAS-RFP, UAS-DenMark::RFP [39 (link)] (BL# 33062) UAS-dpr1, UAS-DIPγ, UAS-DIPδ, dpr10^MI03557[38 (link)] and UAS-RNAi lines were all from Bloomington Stock Center.

Barish S., Nuss S., Strunilin I., Bao S., Mukherjee S., Jones C.D, & Volkan P.C. (2018). Combinations of DIPs and Dprs control organization of olfactory receptor neuron terminals in Drosophila. PLoS Genetics, 14(8), e1007560.

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Drosophila Neurogenetics Experimental Setup

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Flies were maintained at 25 °C and raised on the standard cornmeal food under a 12:12-h light/dark cycle. The driver lines, GMR-Gal4 and GAD-Gal4, have been described previously^{40 (link),41 (link)}. The 202508-Gal4 line was obtained from the Vienna Drosophila Resource Center. UAS-mCD8-GFP, UAS-lacZ, UAS-Denmark, UAS-syt-GFP, tubP-Gal80^ts and UAS-shibire^ts were obtained from the Bloomington Drosophila Stock Center.

Chi H., Sun L., Shiu R.H., Han R., Hsieh C.P., Wei T.M., Lo C.C., Chang H.Y, & Sang T.K. (2020). Cleavage of human tau at Asp421 inhibits hyperphosphorylated tau induced pathology in a Drosophila model. Scientific Reports, 10, 13482.

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Drosophila Behavior Imaging Protocols

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Fruit flies (Drosophila melanogaster) were raised at 25°C in 50–60% relative humidity on standard medium containing cornmeal, yeast, glucose, wheat germ, and agar, as described before [9 (link)]. They were maintained under a 12-h light: dark (LD) cycle. In this study, we used R52B10-Gal4 (BDSC stock number 38820), R23E10-Gal4 (49032), R23E10-LexA (52693), R52B10-LexA (52826), UAS-mCD8::GFP (5130), tub-Gal80^ts (7019), UAS-GCaMP6s (42746), UAS-DenMark, syt.eGFP (33064), LexAop-P2X2 (76030), hDeltaC-Gal4 (75925), and vDeltaB, C, D-Gal4 (93172) from the Bloomington Drosophila Stock Center, and NP2320-Gal4 (DGRC stock number 104157) from the Drosophila Genetics Resource Center. UAS-dTrpA1 [12 (link)] was a gift from Dr. Julie H. Simpson. Cha-Gal80 [13 (link)] was from Dr. Takaomi Sakai. UAS-CD4::spGFP1-10, LexAop- CD4::spGFP11 were from Dr. Kristin Scott. UAS-Kir2.1 [14 (link)] was from Dr. Richard A. Baines. R52B10-Gal4, R23E10-Gal4, NP2320-Gal4, and UAS-dTrpA1 are backcrossed at least 5 times to the control strain (w¹¹¹⁸). Male flies were used in all experiments.

Kato Y.S., Tomita J, & Kume K. (2022). Interneurons of fan-shaped body promote arousal in Drosophila. PLOS ONE, 17(11), e0277918.

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