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Tzm bl pta 5659

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The TZM-bl (PTA-5659) is a cell line derived from the HeLa cell line that has been engineered to express high levels of CD4, CXCR4, and CCR5. This cell line is commonly used for assessing HIV-1 infection and replication.

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Lab products found in correlation

Thp 1 tib 202, by American Type Culture Collection (1 mentions) Hek293t crl 11268, by American Type Culture Collection (1 mentions) Jurkat tib 152, by American Type Culture Collection (1 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Poly lactide co glycolide, by Merck Group (1 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions)

2 protocols using tzm bl pta 5659

1

Culturing and Isolating Diverse Cell Lines

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HEK293T (CRL-11268; ATCC), HeLa (CRM-CCL-2; ATCC), and TZM-bl (PTA-5659; ATCC) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone) containing 10% heat-inactivated fetal bovine serum (FBS, 04-001-1; Biological Industries) and penicillin/streptomycin. H9 (HTB-176; ATCC), Jurkat (TIB-152; ATCC), and THP1 (TIB-202; ATCC) cells were purchased from the ATCC and maintained in Roswell Park Memorial Institute 1640 (RPMI-1640) medium (HyClone) with 10% FBS and penicillin/streptomycin. The peripheral blood mononuclear cells (PBMCs) were isolated through Ficoll gradient centrifugation, and the CD4+ T lymphocytes were then purified from the PBMCs with anti-CD4-specific antibody-coated microbeads (Miltenyi Biotec, Germany) according to the manufacturer’s instructions. CD4+ T lymphocytes were maintained in RPMI-1640 medium (HyClone) with 10% FBS and penicillin/streptomycin.
Gao W., Rui Y., Li G., Zhai C., Su J., Liu H., Zheng W., Zheng B., Zhang W., Yang Y., Hua S, & Yu X. (2021). Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses. Frontiers in Immunology, 12, 740713.
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2

Antiretroviral Nanoformulation Development

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Poly(lactide co-glycolide) [PLGA 75:25 lactide:glycolide ratio; Mw 4,000–15,000], poly (vinyl alcohol) (PVA) (M.W. 13,000–23,000), dichloromethane (DCM), acetonitrile (ACN), potassium dihydrogen phosphate (KH2PO4) and Phosphate Buffered Saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pluronic F127 (PF-127) and dimethyl sulfoxide (DMSO) were purchased from D-BASF (Edinburgh, UK) and Fisher BioReagents (Fair Lawn, NJ, USA), respectively. TAF and EVG (~100% purity) were generously provided by Gilead Sciences Inc. (Foster City, CA, USA). LC-MS grade methanol, acetonitrile, formic acid, and trifluoroacetic acid were purchased (Fisher). Cell line TZM-bl (PTA-5659) was purchased from ATCC® (University Boulevard Manassas, VA 20110 USA.) Dulbecco’s High Glucose Modified Eagles Medium (DMEM), fetal bovine serum (FBS), L-Glutamine, trypsin and penicillin-streptomycin (Penstep) solution were purchased from Hyclone™ (Logan, Utah, USA). All reagents were used as received without further purification.
Mandal S., Prathipati P.K., Kang G., Zhao Y., Yuan Z., Fan W., Li Q, & Destache C.J. (2017). Tenofovir alafenamide and elvitegravir loaded nanoparticles for long-acting prevention of HIV-1 vaginal transmission. AIDS (London, England), 31(4), 469-476.
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