Denaturing whole-cell extracts were prepared in10% trichloroacetic acid by agitation with glass beads. Proteins were solubilized in Laemmli sample buffer, separated by SDS-PAGE and analyzed by western blotting. Antibodies were goat polyclonal anti-LexA (1:2000, Santa Cruz), rat polyclonal anti-α-tubulin (1:5000, Bio-rad), mouse monoclonal anti-Flag M2 (1:2000, Sigma) HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000, this laboratory). Secondary antibodies were used at 1:5000 dilution: IRDye 800CW goat anti-mouse IgG (LI-COR), HRP-conjugated goat anti-mouse IgG (Bio-Rad), HRP-conjugated goat anti-rabbit IgG (Bio-Rad), HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rat IgG (Abcam).
Hrp conjugated mouse monoclonal anti flag m2
Manufactured by Merck Group
HRP-conjugated mouse monoclonal anti-Flag M2 is a laboratory reagent used for the detection and purification of proteins tagged with the Flag peptide sequence. It consists of a mouse monoclonal antibody specific to the Flag tag, conjugated to horseradish peroxidase (HRP) enzyme. This allows for the visualization and quantification of Flag-tagged proteins in various immunoassay and affinity purification techniques.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti mbp, by New England Biolabs (4 mentions)
Irdye 800cw goat anti mouse igg, by LI COR (4 mentions)
Hrp conjugated goat anti rabbit igg, by Bio-Rad (2 mentions)
Hrp conjugated goat anti mouse igg, by Bio-Rad (2 mentions)
Mouse monoclonal anti flag m2, by Merck Group (2 mentions)
Anti lexa, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated donkey anti goat igg, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated donkey anti rat igg, by Abcam (2 mentions)
Rabbit polyclonal anti kar2 y 115, by Santa Cruz Biotechnology (2 mentions)
Irdye 680 goat anti rabbit igg, by LI COR (2 mentions)
Mouse monoclonal anti myc, by Abcam (2 mentions)
Odyssey infrared imaging system, by LI COR (2 mentions)
4 protocols using hrp conjugated mouse monoclonal anti flag m2
1
Western Blot Analysis of Protein Extracts
2
Western Blot Analysis of Protein Extracts
3
Detecting Protein Interactions by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Denaturing whole-cell extracts were prepared in 10% trichloroacetic acid with agitation in the presence of glass beads. Precipitated proteins were solubilized in Laemmli sample buffer and appropriate amounts of protein were separated by SDS-PAGE and analyzed by western blotting. Antibodies were mouse monoclonal anti-myc (1/2000, Abcam), rabbit polyclonal anti-Kar2 (y-115) (1/2000, Santa Cruz), HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB). Secondary antibodies were used at 1/5000: IRDye 800CW goat anti-mouse IgG, IRDye 680 goat anti-rabbit IgG. Western blots were revealed using the Li-COR Bioscience Odyssey infrared imaging system.
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Detecting Protein Interactions by Western Blot
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