For our sample, we purchased doxorubicin (D1515) and kirenol (52659-56-0) from Sigma-Aldrich Co. (St. Louis, MO, USA). A terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining kit (Roche, cat. no. 11684817910), antibodies against pAKT (# 44-621G), AKT (# 44-609G), pPI3K (#PA5-104853), PI3K (# PA5-29220), BCL2 (# PA5-27094), PARP (PA5-16452), HO-1 (# PA5-77833), NQO1 (# PA5-82294), pNrf2 (# PA5-105664), β-actin (# PA5-78716), and Lamin B1 (# PA5-19468), ANP (# 711569), MMP9 (PA5-13199), MMP2 (PA5-85197), and rhodamine phalloidin (R415) were sourced from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Furthermore, we purchased anti-cleaved caspase-3 (ab32042) and Anti-BNP antibody (ab19645) from Abcam (Branford, CT, USA) and Lipofectamine 2000 (11668027) and Nrf2 siRNA from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). We also procured 3-nitrotyrosine (3-NT, 1:3000 dilutions, Millipore, Billerica, MA), 4-hydroxy-2-nonenal (4-HNE, Alpha Diagnostic International, San Antonio, TX, USA), and plasminogen activator inhibitor-1 (PAI-1, BD Biosciences, San Jose, CA, USA).
Anti bnp antibody ab19645
Manufactured by Abcam
Sourced in United States, China
Anti-BNP antibody (ab19645) is a laboratory research tool used to detect and measure levels of the protein B-type natriuretic peptide (BNP). BNP is a biomarker that can provide information about heart function. This antibody can be used in various immunoassay techniques to quantify BNP in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions)
Nrf2 sirna, by Thermo Fisher Scientific (1 mentions)
3 nitrotyrosine 3 nt, by Merck Group (1 mentions)
Pai 1, by BD (1 mentions)
4 hydroxy 2 nonenal 4 hne, by Alpha Diagnostic (1 mentions)
Doxorubicin d1515, by Merck Group (1 mentions)
Tunel staining kit, by Roche (1 mentions)
Complete freund s adjuvant, by Merck Group (1 mentions)
Bx51 light microscope, by Olympus (1 mentions)
Pcr primers, by GenScript (1 mentions)
Ab6671, by Abcam (1 mentions)
Hematoxylin and eosin h e stain kit, by Solarbio (1 mentions)
Ab34710, by Abcam (1 mentions)
Ab7778, by Abcam (1 mentions)
Ab92486, by Abcam (1 mentions)
Phospho nf κb p65 ser536 93h1 rabbit mab3033, by Cell Signaling Technology (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
3 protocols using anti bnp antibody ab19645
1
Doxorubicin and Kirenol Cytotoxicity Assay
2
Molecular Analysis of Cardiac Biomarkers
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The equipment used in this study included an Olympus BX51 light microscope (Olympus Corporation, Tokyo, Japan); complete Freund’s adjuvant (Sigma-Aldrich, St. Louis MO, USA); reverse transcription kit (K1699) (Thermo Fisher Scientific, Waltham, MA, USA); a fluorescence-based quantitative polymerase chain reaction (PCR) kit (RR420A) (TaKara, Tokyo, Japan); PCR primers (GenScript, Nanjing, China); anti-BNP antibody (ab19645) (Abcam, Cambridge, UK); anti-NPR-A antibody (GTX109810) (Genetex, Irvine, CA, USA); anti-GAPDH antibody (ab9485) (Abcam, Cambridge, UK); secondary antibody (ab6789) (Abcam, Cambridge, UK); ECL kit (34094) (Thermo Fisher Scientific, Waltham, MA, USA). Other reagents used were obtained locally and were of pure analytical grade.
3
Investigation of AMPK Signaling in Inflammation
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Sodium Houttuyfonate, Hematoxylin and Eosin (HE) stain kit and Mallory’s trichrome stain kit were purchased from Solarbio Science & Technology (Shanghai, China). The antibodies phospho-AMPKα (Thr172)(40H9)Rabbit mAb#2535, AMPKα(D63G4) Rabbit mAb#5832, NF-κB p65 (D14E12) XP® Rabbit mAb #8242, Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 were purchased from Cell Signaling Technology (Danvers, MA, United States). The antibodies β-Actin (4D3) monoclonal mouse antibody# BS6007M was purchased from Bioworld Technology (Nanjing, China). The antibodies IL-6 mouse Antibody#53324 was purchased from R&D System Technology (Minneapolis, MN, United States). The antibodies tumor necrosis factor (TNF)-α#Rabbit ab6671, Rabbit polyclonal to TGFβ1#ab92486, Rabbit polyclonal to Collagen I#ab34710, and Rabbit polyclonal to Collagen III#ab7778 and Rabbit monoclonal [EPR16891] to GAPDH, anti-BNP antibody ab19645 were purchased from Abcam (Shanghai, China). Horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG antibodies and anti-mouse IgG antibodies were purchased from Biosharp (Hefei, China). Cell counting kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, United States). SiAMPKα was bought from Riobio technologies (Guangzhou, China). Lipofectamine® 2000 reagent was bought from Invitrogen (Carlsbad, CA, United States).
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