Anti fem1b

Manufactured by Proteintech

Anti-FEM1B is a primary antibody that specifically recognizes the FEM1B protein. FEM1B is a protein that plays a role in apoptosis and cell survival. The Anti-FEM1B antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the FEM1B protein.

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Lab products found in correlation

Anti beta actin, by MP Biomedicals (2 mentions) Anti fnip1, by Abcam (2 mentions) Anti ha tag, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (2 mentions) α tomm20, by Merck Group (1 mentions) Anti myogenin, by Developmental Studies Hybridoma Bank (1 mentions) Anti myosin, by Developmental Studies Hybridoma Bank (1 mentions) Anti gfp, by Abcam (1 mentions)

2 protocols using anti fem1b

Antibody Validation for Immunoprecipitation

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The following antibodies were used in this study: anti-FLAG (Clone M2, Sigma-Aldrich, F1804), anti-CUL2 (Bethyl, A302-476A), anti-FNIP1 (Abcam, ab134969), anti-FEM1B (Proteintech, 19544-1-AP), anti-beta-ACTIN (MP Biomedicals, clone C4, 691001), α-TOMM20 (Sigma-Aldrich, HPA011562), BEX2 Antibody (C-12, Santa Cruz Biotechnology, sc-398486) anti-HA-Tag (C29F4, Cell Signaling Technology (CST), 3724), anti-FLAG DYKDDDDK Tag (CST, 2368), and anti-NRF2 (D1Z9C, CST, 12721). For the endogenous 3xFLAG-FEM1B immunoprecipation, as BEX2 runs close to the antibody light chain, we used Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (Jackson ImmunoResearch Laboratories, 115-035-008)

Manford A.G., Mena E.L., Shih K.Y., Gee C.L., McMinimy R., Martínez-González B., Sherriff R., Lew B., Zoltek M., Rodríguez-Pérez F., Woldesenbet M., Kuriyan J, & Rape M. (2021). Structural basis and regulation of the reductive stress response. Cell, 184(21), 5375-5390.e16.

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Antibody Panel for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: anti-Flag (Clone M2, Sigma-Aldrich, F1804), anti-CUL2 (Bethyl, A302-476A), anti-FNIP1 (Abcam, ab134969), anti-FNIP1 (Abcam, 215725), anti-GFP (Abcam, ab6556), anti-FEM1B (Proteintech, 19544-1-AP), anti-MYOSIN, SARCOMERE (MHC) (Developmental Studies Hybridoma Bank, M20), anti-MYOGENIN (Developmental Studies Hybridoma Bank, F5D) anti-beta-ACTIN (MP Biomedicals, clone C4, 691001), a-TOMM20 (Sigma-Aldrich, HPA011562), anti-HA-Tag (C29F4, Cell Signaling Technology (CST), 3724), anti-Flag DYKDDDDK Tag (CST, 2368), anti-FLCN (D14G9, CST, 3697), anti-KEAP1 (D6B12, CST, 8047), anti-NRF2 (D1Z9C, CST, 12721), anti-CITRATE SYNTHASE (D7V8B, CST, 14309), anti-GAPDH (D16H11, CST, 5174), anti-Phospho-AMPKa (Thr172) (40H9, CST, 2535), anti-AMPKa (D5A2, CST, 5831), anti-Phospho-p70 S6 KI-NASE (Thr389) (108D2, CST, 9234), anti-p70 S6 KINASE (49D7, CST, 2708), anti-Phospho-Acetyl-CoA CARBOXYLASE (Ser79) (CST, 3661) , and anti-Acetyl-CoA CARBOXYLASE (C83B10, CST, 3676).

Manford A.G., Rodríguez-Pérez F., Shih K.Y., Shi Z., Berdan C.A., Choe M., Titov D.V., Nomura D.K, & Rape M. (2020). A Cellular Mechanism to Detect and Alleviate Reductive Stress. Cell, 183(1).

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