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L7647

Manufactured by Merck Group
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The L7647 is a laboratory equipment product from Merck Group. It is designed for general laboratory applications. The core function of the L7647 is to provide a platform for various analytical and experimental procedures. Detailed specifications and intended use are not available.

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Lab products found in correlation

Lysozyme, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Anti cd3 clone 2c11, by BD (1 mentions) Easysep cd8 t cell negative selection kit, by STEMCELL (1 mentions) Rpmi 1640 medium with l glutamine, by Lonza (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hepes, by Lonza (1 mentions)

2 protocols using l7647

1

Preparation of Protein Solutions

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Standard protein solutions of hemoglobin (L7647, Sigma), concanavalin A (H2500, Sigma), and lysozyme (62970, Sigma Aldrich) were prepared by dissolving lyophilized powder in water (18.2 MΩ) at the concentrations stated.
Corujo M.P., Sklepari M., Ang D.L., Millichip M., Reason A., Goodchild S.C., Wormell P., Amarasinghe D.P., Lindo V., Chmel N.P, & Rodger A. (2018). Infrared absorbance spectroscopy of aqueous proteins: Comparison of transmission and ATR data collection and analysis for secondary structure fitting. Chirality, 30(8), 957-965.
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2

CD8+ T Cell Activation and PD-L1 Inhibition

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The spleen and lymph nodes of WT and CD80-KO mice were harvested at 6–12 weeks of age. The cells were activated with concanavalin A (ConA, 5 μg/mL, L7647, Sigma-Aldrich) for 48 hours. Following activation, CD8+ T cells were purified from the whole cell population (EasySep CD8+ T cell negative selection kit, Stem Cell Technologies) and were incubated with plate-bound PD-L1/Fc or recombinant human IgG1/Fc (control/Fc) fusion proteins (R&D Systems) for 48 hours in the presence of anti-CD3 (clone 2C11, BD Biosciences) in ConA-conditioned media (RPMI 1640 medium with L-glutamine and 25 mM HEPES (Lonza) with 10% FBS (Gibco), 1 U/mL penicillin (Gibco), and 1 μg/mL streptomycin (Gibco)). Live cells were counted by Trypan blue (Millipore) exclusion using a hemocytometer.
Rollins M.R, & Gibbons Johnson R.M. (2017). CD80 Expressed by CD8+ T Cells Contributes to PD-L1-Induced Apoptosis of Activated CD8+ T Cells. Journal of Immunology Research, 2017, 7659462.
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