A GAS5 over-expression plasmid, null control, small interfering RNA (siRNA) for GAS5 and negative control (siNC) were designed by GenePharma (Shanghai, China). The siRNA sequences are shown in Table 1 of the Supplementary File , available at Carcinogenesis Online. We stably overexpressed GAS5 in T24 cells by transducing the cells with lentivirus (GenePharma, Shanghai, China). The cells were screened with puromycin for 4 weeks. Stable transfectants were used for animal experiments. The miR-18a-5p mimic, mimic control, miR-18a-5p inhibitor and inhibitor control were purchased from RiboBio (Guangzhou, China). Transfection of T24 and EJ cells was performed with Lipo8000™ transfection reagent (Biyuntian Biotechnology, Shanghai, China). After 48 h of transfection, T24 and EJ cells were collected for further experiments.
Mir 18a 5p mimic
Manufactured by RiboBio
Sourced in China
MiR-18a-5p mimic is a laboratory product used for RNA research. It is a synthetic RNA molecule designed to mimic the function of the miR-18a-5p microRNA. The core function of the MiR-18a-5p mimic is to serve as a research tool for studying the biological role and target interactions of the miR-18a-5p microRNA.
Automatically generated - may contain errors
Lab products found in correlation
Mir 18a 5p inhibitor, by RiboBio (4 mentions)
Inhibitor control, by RiboBio (3 mentions)
Mimic control, by RiboBio (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Lipo8000 transfection reagent, by Beyotime (1 mentions)
Lentivirus, by GenePharma (1 mentions)
Pcdna3.1 vector, by Genechem (1 mentions)
Smad2 plasmid, by RiboBio (1 mentions)
Control plasmid, by Santa Cruz Biotechnology (1 mentions)
Pcdna3, by RiboBio (1 mentions)
Si control, by RiboBio (1 mentions)
4 protocols using mir 18a 5p mimic
1
Evaluating GAS5 Regulation in Bladder Cancer
2
Overexpression of lncRNA CASC2 in HFLSs
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For lncRNA CASC2 over-expression, lncRNA CASC2 plasmid was used. The lncRNA CASC2 sequence was synthesized based on the lncRNA CASC2 sequence and then sub-cloned into the pcDNA3.1 vector (lncRNA CASC2-plasmid; Shanghai GeneChem Co., Ltd.). The empty pcDNA3.1 vector was used as a control (control plasmid). The control plasmid, lncRNA CASC2 plasmid, mimic control (cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p mimic (cat. no. miR10000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), inhibitor control (cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p inhibitor (cat. no. miR20000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), control small interfering (si)RNA, and BTG3 siRNA (Santa Cruz Biotechnology, Inc.) were transfected into HFLSs using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h referring to the manufacturer’s protocol. Subsequently, RNA was extracted for qRT-PCR analysis, and Western blotting was adapted to evaluate protein expression.
3
Investigating miR-18a-5p and Smad2 in hHSFs
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hHSFs were transfected with 100 nM miR-18a-5p inhibitor (5'-CUAUCUGCACUAGAUGCACCUUA-3'; Guangzhou RiboBio Co., Ltd.), 100 nM inhibitor control (5'-UUCUCCGAACGUGUCACGUTT-3'; Guangzhou RiboBio Co., Ltd.), 100 nM miR-18a-5p mimic (sense, 5'-UAAGGUGCAUCUAGUGCAGAUAG-3' and anti-sense, 5'-AUCUGCACUAGAUGCACCUUAUU-3'; Guangzhou RiboBio Co., Ltd.), 100 nM mimic control (sense, 5'-UUCUCCGAACGUGUCACGUTT-3' and anti-sense, 5'-ACGUGACACGUUCGGAGAATT-3'; Guangzhou RiboBio Co., Ltd.), 1 µg Smad2-plasmid (cat. no. sc-421525-ACT; Santa Cruz Biotechnology, Inc.), 1 µg control-plasmid (cat. no. sc-108083; Santa Cruz Biotechnology, Inc.) or 100 nM miR-18a-5p mimic + 1 µg Smad2-plasmid using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) in line with the manufacturer's protocol. At 48 h after transfection, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine transfection efficiency.
4
Investigating CASC2 and miR-18a-5p in Melanoma
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A375 cells were seeded onto a 60-mm dish and cultured for 24 h. To evaluate the roles of CASC2 and miR-18a-5p on MM, cells were transiently transfected with pc-CASC2, pcDNA3.1, si-CASC2, si-control, miR-18a-5p inhibitor, inhibitor control, miR-18a-5p mimic, mimic control, or pc-RUNX1, respectively (RiboBio Corp., Guangzhou, P.R. China) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). To further evaluate the relationship of CASC2 and miR-18a-5p, cells were transfected with pc-CASC2, pc-CASC2 + miR-18a-5p mimic, or pc-CASC2 + mimic control, respectively. After treatment for 48 h, cells with various treatments were subjected to the following experiments.
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