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Anti eif4b

Manufactured by Cell Signaling Technology
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Anti-eIF4B is a primary antibody product that detects the eIF4B protein. eIF4B is a eukaryotic translation initiation factor that plays a role in the regulation of protein synthesis.

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Lab products found in correlation

Anti 4e bp1, by Cell Signaling Technology (4 mentions) Anti akt, by Cell Signaling Technology (3 mentions) Anti phospho eif4b, by Cell Signaling Technology (3 mentions) Anti phospho 4ebp1, by Cell Signaling Technology (3 mentions) Anti β actin, by Merck Group (2 mentions) Anti pim1, by Cell Signaling Technology (2 mentions) Anti s6, by Cell Signaling Technology (2 mentions) Anti mtor, by Cell Signaling Technology (2 mentions) Anti phospho akt, by Cell Signaling Technology (2 mentions) Anti phospho s6, by Cell Signaling Technology (2 mentions) Anti irs 1, by Merck Group (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Azd5363, by Adooq (1 mentions) Anti phopho s6 ser 235 236, by Cell Signaling Technology (1 mentions) Bkm 120, by Adooq (1 mentions) Anti phospho eif2α, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Anti phospho rps6, by Cell Signaling Technology (1 mentions) Anti phospho rps6s240 244, by Cell Signaling Technology (1 mentions) Anti s6k1, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

EIF4B (6 citations) Anti-phospho-eIF4B (4 citations)

4 protocols using anti eif4b

1

Antibody and Kinase Inhibitor Validation

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The following antibodies were purchased from Cell Signaling Technology anti-Pim1 (Cat#2907), anti-S6(Cat#9202), anti-phopho-S6 (Ser 235/236 Cat#4856), anti-EDC3 (Cat #14495), anti-phospho-GSK3B, (Ser9, Cat#9336), anti-phopho-IRS1 S1101 (Cat#2385), anti-phospho-eIf4B S406 (Cat #5399), anti-eIF4B (Cat#3592), anti-AKT (Cat#9272), and anti-phopho-AKT S473 (Cat #4058), anti- phosphor 4E-BP1 (T37/46) (Cat#2855), anti-4E-BP1 (Cat#9644), c-Myc (Cat# 9402). The anti-phospho-EDC3 (S161 Cat#600-401-J38) was purchased from Rockland Antibodies while the HRP conjugated anti-β-actin (Cat#A3854) was from Sigma. Anti-IRS1 (Cat #06-248) was obtained from Merck Millipore. Antibodies bought from Santa Cruz Biotechnology included anti-EDC3 (Cat# sc-365024) and anti-GAPDH HRP (Cat. #0411). The kinase inhibitors BKM 120 (Cat# A11016) and AZD5363 (Cat#A11759) were purchased from Adooq Bioscience. VBT5445 was purchased from Chembridge.
Gnawali G.R., Okumura K., Perez K., Gallagher R., Wulfkuhle J., Petricoin E.F., Padi S.K., Bearss J., He Z., Wang W, & Kraft A.S. (2022). Synthesis of 2-Oxoquinoline Derivatives as Dual Pim and mTORC Protein Kinase Inhibitors. Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, 31(7), 1154-1175.
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2

Comprehensive Analysis of mTOR Pathway

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Following infection and other treatments, total cell lysates were prepared for SDS-PAGE and Western blotting as described [72 (link)]. Primary antibodies anti-mTOR (#2983), anti-phospho-S6K1 (T389; #9234), anti-phospho-RPS6 (S235/236; #2211), anti-phospho-RPS6 (S240/244; #5364), anti-phospho-eIF4B (S422, #3591), anti-eIF4B (#3591), anti-phospho-4E-BP1 (T37/46; #2855), anti-4E-BP1 (#9644), anti-phospho-eIF2α (S51; #3398), anti-eIF2α (#2103), anti-PABCP1 (#4992), and β-actin (#3700) were purchased from Cell Signaling Technologies; anti-phospho-PKR (EIF2AK2) (T451; #07–886) was obtained from Millipore; anti-RPS6 (#sc-74459), anti-S6K1 (#sc-230), and anti-PKR (EIF2AK2) (#sc-6282) were acquired from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-linked goat anti-rabbit and goat anti-mouse IgG secondary antibodies were purchased from Sigma-Aldrich.
Chaparro V., Leroux L.P., Masvidal L., Lorent J., Graber T.E., Zimmermann A., Arango Duque G., Descoteaux A., Alain T., Larsson O, & Jaramillo M. (2020). Translational profiling of macrophages infected with Leishmania donovani identifies mTOR- and eIF4A-sensitive immune-related transcripts. PLoS Pathogens, 16(6), e1008291.
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3

Molecular Signaling Pathways in Neuropsychiatry

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Escitalopram oxalate (Pangbourne, UK), and paroxetine HCl (Holzkirchen, DE) were gifts from Sandoz. The antibodies for the Western blot analyses were obtained from the following sources: anti-phospho-mTOR (Ser2448, #2971), anti-mTOR (#2972), anti-phospho-Akt (Ser473, #9271), anti-Akt (#9272), anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204, #9101), anti-p44/42 MAPK (ERK1/2, #4695), anti-phospho-4E-BP-1 (Thr37/46, #2855), anti-4E-BP-1 (#9452), anti-phospho-eIF4B (Ser422, #3591), anti-eIF4B (#3592), anti-phospho-S6 (Ser240/244, #2251), anti-S6 (#2217), anti-phospho-p70S6 K (Thr389, #9205), and anti-p70S6 K (#9202) from Cell Signaling Technology (Beverly, MA, USA); anti-BDNF (sc-546) and goat anti-rabbit IgG-horseradish-peroxide conjugates (sc-2004) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); and monoclonal anti-α-tubulin (T9026) and anti-mouse IgG peroxidase conjugates (A4416) from Sigma (St. Louis, MO, USA).
Seo M.K., Choi C.M., McIntyre R.S., Cho H.Y., Lee C.H., Mansur R.B., Lee Y., Lee J.H., Kim Y.H., Park S.W, & Lee J.G. (2017). Effects of escitalopram and paroxetine on mTORC1 signaling in the rat hippocampus under chronic restraint stress. BMC Neuroscience, 18, 39.
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4

Antibody and Inhibitor Reagents for Signaling Studies

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The following antibodies were purchased from Cell Signaling Technology: Anti-Pim-1 (Cat#3247), anti-Pim2 (Cat#4730), anti-Pim3 (Cat#4165) anti-MET (Cat#8198), anti-phospho-MET (Cat#3077), anti-BAD (Cat#9239), anti-phospho-BAD (Cat#5284), anti-eIF4B (Cat#3592), anti-phospho-eIF4B (S406, Cat#8151), anti-eIF4G (Cat#2498), anti-eIF4E(Cat#2067), anti-Myc-Tag(Cat#2272), anti-AKT (pan, Cat#4691), anti-phospho-AKT (S473, Cat#4058), anti-phospho-4E-BP1 (Cat#2855), anti-4E-BP1 (Cat#9452), anti-phospho-S6 (Cat#2215), anti-ERK (Cat#9102), anti-phospho-ERK (Cat#9101), anti-Bcl-2(Cat#4223), anti-Bim(Cat#2933), and anti-cleaved PARP(Cat#5625). Anti-β-actin (Cat#A3854), anti-β-tubulin (Cat#T4026), anti-FLAG (Cat#F1804) antibodies were purchased from Sigma. Anti-Mcl-1 antibody (Cat#sc-12756) was from Santa Cruz Biotechnology. A neutralizing antibody against MET was from Abcam (Cat#ab10728). HRP-linked enhanced chemiluminescence (ECL) mouse (Cat#NA931V) and rabbit IgG (Cat#NAV934V) were purchased from GE Healthcare Life Sciences.
The small molecule inhibitors PP242, BEZ235, ABT199, and ABT737 were obtained from Selleck Biochemicals. PHA665752 was from Cayman Chemical. LGB321 was provided by Norvatis. AZD6094 and AZD1208 were provided by AstraZeneca. LY2801653 was from Eli Lilly.
An N., Xiong Y., LaRue A.C., Kraft A.S, & Cen B. (2015). Activation of Pim Kinases Is Sufficient to Promote Resistance to MET Small Molecule Inhibitors. Cancer research, 75(24), 5318-5328.
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