Ifn γ and il 17 elisa kits

The levels of serum hsCRP and IL-6 were measured using hsCRP and IL-6 ELISA kits (R&D), according to the manufacturer’s instructions. The minimum detectable levels were 0.006 ng mL⁻¹ for hsCRP and 1.6 pg mL⁻¹ for IL-6. The levels of serum IFN-γ and IL-17 were measured using IFN-γ and IL-17 ELISA kits (Neobioscience), according to the manufacturer’s instructions. The minimum detectable levels were 3.9 pg mL⁻¹ for IFN-γ and 0.78 pg mL⁻¹ for IL-17. All the experiments were performed in triplicate. The level of Serum cardiac troponin T (cTnT) was measured using a rapid assay kit from Roche Diagnostics (Shanghai, China) as previously described by Metzler (Metzler et al., 2001 (link)). The experiment was also performed in triplicate.

Complete Freund’s adjuvant (CFA) was bought from Sigma (Shanghai, China). Sac/Val and valsartan were purchased from Novartis Pharma AG (Basel, Switzerland). Antibodies against IFN-γ (Cat# sc-8423, RRID: AB_627,778), IL-1β, IL-17 (Cat# sc-374218, RRID: AB_10988239), and β-actin were bought from Santa Cruz (Dallas, USA). Anti-IL-18 antibody was purchased from Arigobio (Hsinchu City, Taiwan). The IL-6 ELISA kit (Cat# M6000B, RRID: AB_2877063) and the hsCRP ELISA kit were purchased from R andD (Minneapolis, USA). The IFN-γ and IL-17 ELISA kits were purchased from Neobioscience (Shenzhen, China). The cTnT quantitative rapid assay kit and collagenase B were bought from Roche Diagnostics (Shanghai, China). The total protein extraction kit, super ECL reagent, and antibody against ROR-γt (Cat# 14-6988-80, RRID: AB_1311291) were bought from Thermo Fisher Scientific Inc. (Hanover Park, USA). Antibodies against NLRP3 (Cat# AG-20B-0014, RRID: AB_2490202), ASC (Cat# AG-25B-0006, RRID: AB_2490440) and caspase-1 (Cat# AG-20B-0042, RRID: AB_2490248) were purchased from AdipoGen. Antibodies against T-bet, sGC-α1, sGC-β1, total-NF-κB p65 and phospho-NF-κB p65 were purchased from ImmunoWay (Plano, United States). FITC-anti-mouse CD4, PE-anti-mouse IFN-γ and allophycocyanin-anti-mouse IL-17 were purchased from eBioscience.