The heterochronic parabiosis model was established 2 weeks after surgery. FMT4000 (PerkinElmer, Boston, MA, USA) was used to confirm that the partners in the model had a common blood circulation. FMT is a noninvasive technique utilising fluorescence, characterised by molecular specificity and sensitivity, and is used for tissue imaging in live animals [15 (link)]. ProSense 750-fluorescence agents (PerkinElmer, Boston, MA, USA) become fluorescent upon activation in the presence of cathepsins (cathepsins B, L, S, and plasmin), but do not fluoresce without any activation. ProSense 750FAST was injected into the old mouse in the heterochronic parabiosis model via the tail vein. The young mouse was imaged using FMT4000 24 hours after injection.
Prosense 750 fluorescence agents
Manufactured by PerkinElmer
Sourced in United States
ProSense 750-fluorescence agents are laboratory reagents designed for fluorescence-based detection and quantification. They are used in various analytical techniques to measure the presence and concentration of specific analytes or targets within a sample.
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Lab products found in correlation
2 protocols using prosense 750 fluorescence agents
1
Fluorescence Imaging of Cathepsin Activation in Heterochronic Parabiosis
2
Fluorescence Imaging of Cathepsin Activation in Heterochronic Parabiosis
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The heterochronic parabiosis model was established 2 weeks after surgery. FMT4000 (PerkinElmer, Boston, MA, USA) was used to confirm that the partners in the model had a common blood circulation. FMT is a noninvasive technique utilising fluorescence, characterised by molecular specificity and sensitivity, and is used for tissue imaging in live animals [15 (link)]. ProSense 750-fluorescence agents (PerkinElmer, Boston, MA, USA) become fluorescent upon activation in the presence of cathepsins (cathepsins B, L, S, and plasmin), but do not fluoresce without any activation. ProSense 750FAST was injected into the old mouse in the heterochronic parabiosis model via the tail vein. The young mouse was imaged using FMT4000 24 hours after injection.
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