Adavosertib

Adavosertib, dabrafenib, trametinib, sorafenib and lenvatinib were purchased from Selleck Chemicals. Adavosertib, dabrafenib, trametinib, sorafenib and lenvatinib were each dissolved in dimethyl sulfoxide (DMSO; Sigma) to a concentration of 10 mmol/L for in vitro experiments. For xenograft experiments, Adavosertib and lenvatinib were diluted in methyl cellulose (Sigma) and distilled water (1:200 w/v) to a concentration of 12 mg/ml and stored at −80°C until use. dabrafenib and trametinib were dissolved in 0.5% (hydroxypropyl)methyl cellulose (Sigma), 0.2% Tween 80 (Sigma), and distilled water to concentrations of 8 mg/ml and 0.16 mg/ml, respectively, and stored at −80°C until use. sorafenib was dissolved in 50% Kolliphor EL (Sigma) and 50% ethanol (Sigma) to a concentration of 57.6 mg/mL and stored at −80°C; it was further dissolved at a final concentration of 14.4 mg/mL in water before in vivo use.