Pe conjugated antihuman hla a2 clone bb7

Manufactured by BioLegend

Sourced in United States

PE-conjugated antihuman HLA-A2 (clone BB7.2) is a monoclonal antibody that binds to the HLA-A2 allele expressed on the surface of human cells. It is conjugated with the fluorescent dye Phycoerythrin (PE).

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Lab products found in correlation

Human trustain fcx block, by BioLegend (2 mentions) Accuri 6 plus, by BD (2 mentions) Clone w6 32, by BioLegend (1 mentions)

Close spelling variants of this product

PE-HLA-A2 Clone BB7.2

2 protocols using pe conjugated antihuman hla a2 clone bb7

Cell Surface HLA Profiling of Tumor Organoids

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The following antibodies were
used to analyze the cell surface expression of HLA-A2 and HLA-A, HLA-B,
and HLA-C: PE-conjugated antihuman HLA-A2 (clone BB7.2, BioLegend
343306 San Diego, CA), PE-conjugated antihuman HLA-A, HAL-B, and HLA-C
(clone W6/32, BioLegend 311406, San Diego, CA), and Human TruStain
FcX block (BioLegend B247182, San Diego, CA).
The data were
acquired using a BDLSR Fortessa flow cytometer. Flow cytometric analysis
of renal cell carcinoma and bladder tumor-derived organoids was performed
using a BD Accuri 6 plus (BD Biosciences) and analyzed with FlowJo
software (Tree Star, Ashland, OR).

Feola S., Haapala M., Peltonen K., Capasso C., Martins B., Antignani G., Federico A., Pietiäinen V., Chiaro J., Feodoroff M., Russo S., Rannikko A., Fusciello M., Koskela S., Partanen J., Hamdan F., Tähkä S.M., Ylösmäki E., Greco D., Grönholm M., Kekarainen T., Eshaghi M., Gurvich O.L., Ylä-Herttuala S., M. Branca R.M., Lehtiö J., Sikanen T.M, & Cerullo V. (2021). PeptiCHIP: A Microfluidic Platform for Tumor Antigen Landscape Identification. ACS Nano, 15(10), 15992-16010.

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HLA Surface Expression Analysis

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The following antibodies were used to analyse the cell surface expression of HLA-A2 and HLA-A, B, and C: PE-conjugated anti-human HLA-A2 (clone BB7.2, BioLegend 343306 San Diego, CA, USA), PE-conjugated anti-human HLA-A, B, and C (clone W6/32, BioLegend 311406, San Diego, CA, USA), and Human TruStain FcX block (BioLegend B247182, San Diego, CA, USA).
The data were acquired using a BDLSR Fortessa Flow Cytometer. Flow cytometric analysis of renal cell carcinoma and bladder tumour-derived organoids was performed using a BD Accuri 6 plus (BD Biosciences) and analysed with FlowJo software (Tree Star, Ashland, OR, USA).

Feola S., Haapala M., Peltonen K., Capasso C., Martins B., Antignani G., Federico A., Pietiäinen V., Chiaro J., Feodoroff M., Rannikko A., Fusciello M., Koskela S., Partanen J., Hamdan F., Tähkä S., Ylösmäki E., Greco D., Grönholm M., Kekarainen T., Es-haghi M., Gurvich O.L., Ylä‐Herttuala S., Branca R.M., Lehtiö J., Sikanen T., & Cerullo V. (2021). PeptiCHIP: a novel microfluidic-based chip platform for tumour antigen landscape identification.

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