Log In Sign up
The largest database of trusted experimental protocols

Anti foxp3 percp cy5

Manufactured by BD
Visit Supplier

Anti-FOXP3 PerCP-Cy5.5 is a fluorescent-conjugated antibody that binds to the FOXP3 protein. FOXP3 is a transcription factor that is a marker for regulatory T cells. The PerCP-Cy5.5 fluorescent dye is used for detection and identification of cells expressing FOXP3.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd4 bv650, by BioLegend (2 mentions) Anti cd3 bv875, by BioLegend (2 mentions) Anti il 2 bv510, by BioLegend (2 mentions) Anti tnf α apc, by BioLegend (2 mentions) Anti il10 percp cy5, by BioLegend (2 mentions) Anti cd45 bv605, by BioLegend (2 mentions) Lsrfortessa x 20, by BD (2 mentions) Anti cd8, by BioLegend (2 mentions) Anti ifn γ fitc, by BioLegend (2 mentions)

Close spelling variants of this product

FoxP3 PerCP-Cy5.5 Anti-FoxP3-PerCP PerCP-Cy5.5 Anti-Foxp3

2 protocols using anti foxp3 percp cy5

1

Multicolor Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nine days after the treatment of MC38 tumours with indicated compounds, tumor infiltrating lymphocytes (TILs) were isolated and stained. For stimulation, cells were incubated with cell stimulation cocktail (PMA and Ionomycin) plus protein transport inhibitor (Brefeldin A and Menesin) for 4 hrs. Cells were analysed by multicolour flow cytometry (BD LSR Fortessa X-20). Antibodies: anti-CD45 BV605 (#103140, Biolegend); anti-CD3 BV875 (#100355, Biolegend); anti-CD4 BV650 (#100469, Biolegend); anti-CD8 (#100784, Biolegend); anti-FOXP3 PerCP-Cy5.5 (#563902, BD); anti-TNFα APC (#506308, Biolegend); anti-IFNγ FITC (#505806, Biolegend); anti-IL-2 BV510 (#503833, Biolegend); anti-IL10 PerCP-Cy5.5 (#505028, Biolegend). All analyses were performed using FlowJo_V10.6.1 software (Tree Star). Gating strategy: gate cells exclude dead cells and debris based on cells size, then gate live cells based on Live-Dead NIR negative cells, then gate CD45+ cells, then gate CD45+CD3+ cells, then gate CD45+CD3+CD8+ cells and CD45+CD3+CD4+ cells. Cytokine expression was determined in the populations of CD45+CD3+cells.
Gao Y., Nihira N.T., Bu X., Chu C., Zhang J., Kolodziejczyk A., Fan Y., Chan N.T., Ma L., Liu J., Wang D., Dai X., Liu H., Ono M., Nakanishi A., Inuzuka H., North B.J., Huang Y.H., Sharma S., Geng Y., Xu W., Liu X.S., Li L., Miki Y., Sicinski P., Freeman G.J, & Wei W. (2020). Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy. Nature cell biology, 22(9), 1064-1075.
+ Open protocol
+ Expand
2

Multicolor Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nine days after the treatment of MC38 tumours with indicated compounds, tumor infiltrating lymphocytes (TILs) were isolated and stained. For stimulation, cells were incubated with cell stimulation cocktail (PMA and Ionomycin) plus protein transport inhibitor (Brefeldin A and Menesin) for 4 hrs. Cells were analysed by multicolour flow cytometry (BD LSR Fortessa X-20). Antibodies: anti-CD45 BV605 (#103140, Biolegend); anti-CD3 BV875 (#100355, Biolegend); anti-CD4 BV650 (#100469, Biolegend); anti-CD8 (#100784, Biolegend); anti-FOXP3 PerCP-Cy5.5 (#563902, BD); anti-TNFα APC (#506308, Biolegend); anti-IFNγ FITC (#505806, Biolegend); anti-IL-2 BV510 (#503833, Biolegend); anti-IL10 PerCP-Cy5.5 (#505028, Biolegend). All analyses were performed using FlowJo_V10.6.1 software (Tree Star). Gating strategy: gate cells exclude dead cells and debris based on cells size, then gate live cells based on Live-Dead NIR negative cells, then gate CD45+ cells, then gate CD45+CD3+ cells, then gate CD45+CD3+CD8+ cells and CD45+CD3+CD4+ cells. Cytokine expression was determined in the populations of CD45+CD3+cells.
Gao Y., Nihira N.T., Bu X., Chu C., Zhang J., Kolodziejczyk A., Fan Y., Chan N.T., Ma L., Liu J., Wang D., Dai X., Liu H., Ono M., Nakanishi A., Inuzuka H., North B.J., Huang Y.H., Sharma S., Geng Y., Xu W., Liu X.S., Li L., Miki Y., Sicinski P., Freeman G.J, & Wei W. (2020). Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy. Nature cell biology, 22(9), 1064-1075.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!