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Anti foxp3 percp cy5

Manufactured by BD

Anti-FOXP3 PerCP-Cy5.5 is a fluorescent-conjugated antibody that binds to the FOXP3 protein. FOXP3 is a transcription factor that is a marker for regulatory T cells. The PerCP-Cy5.5 fluorescent dye is used for detection and identification of cells expressing FOXP3.

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2 protocols using anti foxp3 percp cy5

1

Multicolor Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

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Nine days after the treatment of MC38 tumours with indicated compounds, tumor infiltrating lymphocytes (TILs) were isolated and stained. For stimulation, cells were incubated with cell stimulation cocktail (PMA and Ionomycin) plus protein transport inhibitor (Brefeldin A and Menesin) for 4 hrs. Cells were analysed by multicolour flow cytometry (BD LSR Fortessa X-20). Antibodies: anti-CD45 BV605 (#103140, Biolegend); anti-CD3 BV875 (#100355, Biolegend); anti-CD4 BV650 (#100469, Biolegend); anti-CD8 (#100784, Biolegend); anti-FOXP3 PerCP-Cy5.5 (#563902, BD); anti-TNFα APC (#506308, Biolegend); anti-IFNγ FITC (#505806, Biolegend); anti-IL-2 BV510 (#503833, Biolegend); anti-IL10 PerCP-Cy5.5 (#505028, Biolegend). All analyses were performed using FlowJo_V10.6.1 software (Tree Star). Gating strategy: gate cells exclude dead cells and debris based on cells size, then gate live cells based on Live-Dead NIR negative cells, then gate CD45+ cells, then gate CD45+CD3+ cells, then gate CD45+CD3+CD8+ cells and CD45+CD3+CD4+ cells. Cytokine expression was determined in the populations of CD45+CD3+cells.
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2

Multicolor Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nine days after the treatment of MC38 tumours with indicated compounds, tumor infiltrating lymphocytes (TILs) were isolated and stained. For stimulation, cells were incubated with cell stimulation cocktail (PMA and Ionomycin) plus protein transport inhibitor (Brefeldin A and Menesin) for 4 hrs. Cells were analysed by multicolour flow cytometry (BD LSR Fortessa X-20). Antibodies: anti-CD45 BV605 (#103140, Biolegend); anti-CD3 BV875 (#100355, Biolegend); anti-CD4 BV650 (#100469, Biolegend); anti-CD8 (#100784, Biolegend); anti-FOXP3 PerCP-Cy5.5 (#563902, BD); anti-TNFα APC (#506308, Biolegend); anti-IFNγ FITC (#505806, Biolegend); anti-IL-2 BV510 (#503833, Biolegend); anti-IL10 PerCP-Cy5.5 (#505028, Biolegend). All analyses were performed using FlowJo_V10.6.1 software (Tree Star). Gating strategy: gate cells exclude dead cells and debris based on cells size, then gate live cells based on Live-Dead NIR negative cells, then gate CD45+ cells, then gate CD45+CD3+ cells, then gate CD45+CD3+CD8+ cells and CD45+CD3+CD4+ cells. Cytokine expression was determined in the populations of CD45+CD3+cells.
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