Serum free fatty acid levels were analyzed by GC/MS. In brief, the samples were thawed on ice and chloroform methanol solution was added. After ultrasonication, the supernatant was taken. Then 1% sulfuric acid-methanol solution was added and the mixtures were incubated at 80 °C for 30 min for free fatty acid methyl esterification. Thereafter, the methylated fatty acids were extracted by n-hexane and washed by water. The levels of methylated fatty acids were determined by GC/MS using Agilent 7890A/5975C gas chromatography-mass spectrometer with an Agilent DB-WAX capillary column (30 m×0.25 mm ID × 0.25 μm) (Agilent Technologies, CA, USA). In this analysis, 39 free fatty acids of medium and long-chain length (i.e., C6–C24) were quantified. The analysis was performed by Shanghai Applied Protein Technology Inc.
Agilent db wax capillary column
Manufactured by Agilent Technologies
Sourced in United States
The Agilent DB-WAX capillary column is a gas chromatography (GC) column designed for the separation and analysis of polar compounds. It features a polyethylene glycol (PEG) stationary phase, which provides selectivity for polar analytes. The column is suitable for a wide range of applications, including the analysis of alcohols, organic acids, and other polar compounds.
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Lab products found in correlation
2 protocols using agilent db wax capillary column
1
Quantification of Serum Free Fatty Acids
2
Comprehensive GC/MS Analysis of Seminal Plasma Fatty Acids
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The levels of free fatty acids in seminal plasma were examined using gas chromatography coupled with mass spectrometry (GC/MS). In summary, the specimens were defrosted on ice and supplemented with a solution of chloroform and methanol. Following the application of ultrasonication, the liquid portion was gathered. Next, a solution of sulfuric acid‐methanol with a concentration of 1% was introduced, and the mixtures were left to incubate at a temperature of 80°C for a duration of 30 min to facilitate the esterification of free fatty acids. Afterwards, the methylated fatty acids were obtained by extracting them using n‐hexane and then rinsing them with water. GC/MS was utilized to determine the concentrations of methylated fatty acids employing an Agilent 7890A/5975C GC/MS equipped with an Agilent DB‐WAX capillary column (30 m × 0.25 mm ID × 0.25 μm) (Agilent Technologies, CA). The analysis involved quantifying the quantities of 39 free fatty acids with medium and long chains (specifically, C6‐C24). Shanghai Applied Protein Technology conducted this analysis.
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