Reagents were sourced from the following vendors: NS-1 (Cellomics, Inc., Pittsburgh, PA), PC12 (ATCC, Manassas, VA), 2.5S murine NGF (Bachem Inc., Torrance, CA), RNA extraction kit and Turbo DNA-free kit and RETROscript Kit (Ambion Inc., Carlsbad, CA), RT PCR primers (Eurofins MWG Operon, Huntsville, AL); RT2 SYBR Green ROX qPCR Mastermix (QIAGEN, Valencia, CA), MTT (3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich, St. Louis, MO), DMEM and RPMI 1640, HCS CellMask Red™, and Alexa Fluor 488 Donkey anti-rabbit antibody (Invitrogen, Carlsbad, CA), fetal bovine serum (FBS) (Hyclone, Logan, UT), heat inactivated horse serum (Lonza, Walkersville, MD), and L-glutamine and 1% Penicillin/Streptomycin (BioWhittaker, Walkersville, MD), Mouse anti-ChAT and Rabbit anti-M2 antibody (EMD Millipore, Billerica, MA), Goat anti-VAChT (Promega, Madison, WI), Rabbit anti-TrkA (Cell Signaling, Tech., Danvers, MA), and Super Signal Pico Chemiluminescent Substrate (Thermo Fisher Sci., Grand Island, NY).
Dmem and rpmi 1640
Manufactured by Thermo Fisher Scientific
DMEM (Dulbecco's Modified Eagle's Medium) and RPMI 1640 are commonly used cell culture media formulations. They provide the necessary nutrients, minerals, and other components to support the growth and maintenance of various cell types in vitro. These media are designed to maintain the optimal pH, osmolarity, and nutrient balance required for cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Rt2 sybr green rox qpcr mastermix, by Qiagen (2 mentions)
Super signal pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 donkey anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Heat inactivated horse serum, by Lonza (2 mentions)
Rt pcr primers, by Eurofins (2 mentions)
L glutamine and 1 penicillin streptomycin, by Lonza (2 mentions)
Mouse anti chat and rabbit anti m2 antibody, by Merck Group (2 mentions)
Goat anti vacht, by Promega (2 mentions)
Rabbit anti trka, by Cell Signaling Technology (2 mentions)
Hcs cellmask red, by Thermo Fisher Scientific (2 mentions)
2 protocols using dmem and rpmi 1640
1
Neurotrophic Factor Signaling in PC12 Cells
2
Neurotrophic Factor Signaling in PC12 Cells
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Reagents were sourced from the following vendors: NS-1 (Cellomics, Inc., Pittsburgh, PA), PC12 (ATCC, Manassas, VA), 2.5S murine NGF (Bachem Inc., Torrance, CA), RNA extraction kit and Turbo DNA-free kit and RETROscript Kit (Ambion Inc., Carlsbad, CA), RT PCR primers (Eurofins MWG Operon, Huntsville, AL); RT2 SYBR Green ROX qPCR Mastermix (QIAGEN, Valencia, CA), MTT (3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich, St. Louis, MO), DMEM and RPMI 1640, HCS CellMask Red™, and Alexa Fluor 488 Donkey anti-rabbit antibody (Invitrogen, Carlsbad, CA), fetal bovine serum (FBS) (Hyclone, Logan, UT), heat inactivated horse serum (Lonza, Walkersville, MD), and L-glutamine and 1% Penicillin/Streptomycin (BioWhittaker, Walkersville, MD), Mouse anti-ChAT and Rabbit anti-M2 antibody (EMD Millipore, Billerica, MA), Goat anti-VAChT (Promega, Madison, WI), Rabbit anti-TrkA (Cell Signaling, Tech., Danvers, MA), and Super Signal Pico Chemiluminescent Substrate (Thermo Fisher Sci., Grand Island, NY).
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