Nocodazole (HY-13520), paclitaxel (HY-B0015), cisplatin (HY-17394), chloroquine (HY-17589A), doxorubicin (HY-15142) and camptothecin (HY-16560) were all purchased from MedChem Express. The following antibodies were used: anti-CGAS (15102), anti-LC3B (3868), anti-H2AX (7631), anti-LAMP1 (9091), anti-CAT/catalase (12980), anti-VDAC (4661), anti-CALR/calreticulin (12239), anti-phospho-H2AX (9718), anti-GFP (2956), anti–ATG7 (8558), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (7074) or anti-mouse (7076) IgG (all from Cell Signaling Technology); anti-hemagglutinin (HA; H6908), anti-FLAG (F3165) and anti-GAPDH (G9545) (all from Sigma-Aldrich); anti-LC3B (PM036) and anti-LC3B (M152-3) were obtained from Medical & Biological Laboratories; anti-LAMP2 (sc-18822) was purchased from Santa Cruz Biotechnology. GFP-Trap Agarose (gtd-20) was purchased from ChromoTek GmbH. The plasmid containing the DNA encoding human CGAS (F122086), CGAS mutation (site-directed mutation from F122086), LC3B (F102982) and TEME-192 (F100950) were obtained from Changsha Youbio Tech and cDNAs were then subcloned into the pCDH vector (Changsha Youbio Tech, VT1480). YFP-Atg8-family proteins encoding plasmids were a kind gift from Dr. Felix Randow (MRC Laboratory of Molecular Biology, UK). cgas knockout mice (026554) were obtained from Jackson Laboratory.
Hy 16560
Manufactured by MedChemExpress
Sourced in China
HY-16560 is a laboratory equipment product from MedChemExpress. It is designed for general laboratory use, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
2 protocols using hy 16560
1
Molecular Mechanisms of Autophagy Regulation
2
Oral CPT Treatment in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were orally treated with 60 μl of vehicle (0.1% Tween 80) for 3 days before CPT or vehicle treatment. Mice received 1 mg kg−1 of CPT (Med Chem Express, HY-16560, Shanghai, China) or vehicle (0.1% Tween 80) daily until the end of experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!