We extracted total RNA from cells using the RNA isolator Total RNA Extraction Reagent (Vazyme, Nanjing, Jiangsu, China) according to the manufacturer’s instructions. Next, we reverse-transcribed RNA into cDNA using a HiScript III RT SuperMix qPCR kit (R323-01, Vazyme, Nanjing, China). We performed RT-qPCR using SYBR qPCR SuperMix Plus (Novoprotein Scientific Inc., Shanghai, China) on an Applied Biosystems 7500 Real-Time PCR system. We normalized the relative expression of genes to 18S ribosomal RNA (18srRNA) expression and analyzed it using the 2−ΔΔCT method. The primers used in this study were:
LINC01526-F, 5′-GGAAGGTCCTGCCCTTTGTT-3′; LINC01526-R, 5′-CTGTCCTATTCAGTGGGGGC-3′;
TARBP2-F, 5′-GCCTGAGGACATTCCGGTTT-3′; TARBP2-R, 5′-TCACTGTGTACTCCGGCAAC-3′;
GNG7-F, 5′-AAGCTCTCTGAACAACGGGG-3′; GNG7-R, 5′-CGCTCAATCCCGGCTTCTAT-3′;
CDKN2A-F, 5′-CCAGAGGCAGTAACCATGCC-3′; CDKN2A-R, AAACTACGAAAGCGGGGTGG-3′;
GSDMD-F, 5′-CATGGTTCTGGAAACCCCGT-3′; GSDMD-R, 5′-CCACACTCAGCGAGTACACA-3′
POLG-F, 5′-AGGGGCATTGTTGCTTGTTG-3′; POLG-R, 5′-ACTGCCTTGGAGCAGGTTTAT-3′;
18srRNA-F, 5′-AAACGGCTACCACATCCAAG-3′; 18srRNA-R, 5′-CCTCCAATGGATCCTCGTTA-3′.