The cells were seeded into cell culture plates and stained with JC1 staining solution (40705ES03, Yeasen Biotechnology, Shanghai, China) following the instruction manual. The fluorescence was observed with Leica DMi8 fluorescence microscope. Reactive Oxygen Species were detected using the Reactive Oxygen Species Assay Kit (50101ES01, Yeasen Biotechnology, Shanghai, China), and visualized with Leica DMi8 fluorescence microscope. The quantification of fluorescence results was done using the Image J software. Additionally, ER-Tracker Green (BODIPY FL Glibenclamide) (40763ES20, Yeasen Biotechnology, Shanghai, China), ER-Tracker Blue-White DPX (40761ES50, Yeasen Biotechnology, Shanghai, China) and MitoTracker Red CMXRos (40741ES50, Yeasen Biotechnology, Shanghai, China) were used to stain the endoplasmic reticulum or mitochondria of live cells. The staining was visualized using laser scanning confocal microscopy (Leica, TCS SP8 DIVE, Germany), and the co-localization was quantified using the co-localization Finder plugin of ImageJ software. The experimental protocols were conducted as per the standards.
Er tracker green bodipy fl glibenclamide
Manufactured by Yeasen
Sourced in China
ER-Tracker Green (BODIPY FL Glibenclamide) is a fluorescent probe that specifically labels the endoplasmic reticulum (ER) in live cells. It is a derivative of the antidiabetic drug glibenclamide, which binds to the sulfonylurea receptor on the ER membrane. The BODIPY FL fluorescent dye is attached to the glibenclamide molecule, allowing for visualization of the ER structure in green fluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Tcs sp8 dive, by Leica camera (1 mentions)
Reactive oxygen species assay kit, by Yeasen (1 mentions)
Jc1 staining solution, by Yeasen (1 mentions)
Er tracker blue white dpx, by Yeasen (1 mentions)
Mitotracker red cmxros, by Yeasen (1 mentions)
Leica dmi8 fluorescence microscope, by Leica camera (1 mentions)
N n diisopropylethylamine, by J&K Scientific (1 mentions)
Nf200, by Proteintech (1 mentions)
Mito tracker green, by Beyotime (1 mentions)
Lysotracker green, by Beyotime (1 mentions)
2 protocols using er tracker green bodipy fl glibenclamide
1
Multimodal Imaging of Cellular Organelles
2
Intracellular Labeling and Organelle Imaging
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ICG were purchased from Aladdin Scientific; ICG-NHS and N,N-Diisopropylethylamine (DIPEA, 99 %) were purchased from J&K Scientific; BDA (D1956) were purchased from Molecular Probes; Colchicine was purchased from GlpBio; Anti S100β, anti NF200, anti β-Tubulin Polyclonal antibody were purchased from Proteintech; Dimethyl sulfoxide (DMSO, 99.9 %) and N,N-Dimethylformamide (DMF, 97 %) were purchased form LeYan; DMEM were purchased from Thermo Fisher; DAPI, Lyso-Tracker Green (C1047S) and Mito-Tracker Green (C1048) were purchased from Beyotime; ER-Tracker Green (BODIPY® FL Glibenclamide) was purchased from YEASEN Biotech; PC12 cells were purchased from the Chinese Academy of Sciences. The cells were cultivated in a DMEM medium in a humidified incubator with 5 % CO2 at 37 °C. All reagents were used as received without further purification. Deionized water was used in all experiments (18.2 MΩ cm−1). The 33-gauge 5 μL microsyringe was purchased from Hamilton.
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