A total of 6 mL peripheral blood sample was collected, of which 3 mL blood was collected in a fluoride vacutainer tube to separate the serum from the blood and 3 mL blood was collected in an ethylenediaminetetraaceticacid (EDTA) vacutainer tube for DNA separation. Serum samples were used to evaluate the fasting blood sugar (FBS), postprandial blood glucose (PPBG), GCT and OGTT values (Al-Daghri et al., 2014 (link)). The body mass index (BMI) was calculated as weight/height2 (kg/m2). The Bio-Rad DNA Extraction Kit was used to extract DNA from the collected peripheral blood leucocytes, and the DNA was stored at −80 °C until use.
Dna extraction kit
Manufactured by Bio-Rad
The DNA Extraction Kit is a laboratory equipment designed to isolate and purify DNA from a variety of biological samples. It provides a standardized procedure for extracting high-quality DNA that can be used in various downstream applications, such as PCR, sequencing, and genetic analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dna extraction kit
1
Blood Sampling and Analysis for Diabetes
2
ChIP Assay of miR-518a-5p Promoter
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A ChIP assay kit (#P2078, Beyotime, Shanghai, China) was used according to the manufacturer's protocols. HTR8/SVneo cells were cross-linked with 1% formaldehyde for 12 min and sonicated into DNA fragments of 200 and 1000 bp. Cell lysates were incubated with the NF-κB antibodies (Abcam) that were coated with protein A/G beads at 4°C overnight. The goat-anti-rabbit IgG served as a negative control. The immunocomplexes that were bound to protein A/G beads were then eluted with elution buffer to remove the nonspecific binding. Samples were treated with 5 M NaCl and heated at 65°C overnight to eliminate histone-DNA crosslinks. Next, proteinase K was added followed by incubation at 45°C for 2 h. A DNA Extraction Kit (BIO-RAD) was used to purify the bound DNA fragments. Products were finally analyzed by real-time PCR using the primers specific to miR-518a-5p promoter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!