2 7 dichlorofluorescin diacetate h2dcf da

Manufactured by Thermo Fisher Scientific

Sourced in United States

2',7'-Dichlorofluorescin diacetate (H2DCF-DA) is a fluorogenic compound that can be used to measure intracellular reactive oxygen species (ROS) levels. It is a cell-permeable, non-fluorescent precursor that becomes fluorescent upon oxidation.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

2’,7’-dichlorofluorescin diacetate (17 citations) H2DCF (12 citations) H2DCF-diacetate (2 citations)

4 protocols using 2 7 dichlorofluorescin diacetate h2dcf da

Oxidative Stress Modulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

2,2,6,6-Tetramethylpiperidine 1-oxyl (TEMPO), 4-methoxy-2,2,6,6-tetramethyl-1-piperidinyloxy (4-methoxy-TEMPO), Williams’ medium E, Dulbecco’s Modified Eagle’s Medium (DMEM), JNK inhibitor SP600125, ERK inhibitor U0126, p38 MAPK inhibitor SB203580, N-acetylcysteine (NAC), and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2′,7′-Dichlorofluorescin diacetate (H₂DCF-DA), and penicillin–streptomycin antibiotic were obtained from Life Technologies (Grand Island, NY, USA).

Zhang Z., Ren Z., Chen S., Guo X., Liu F., Guo L, & Mei N. (2017). ROS generation and JNK activation contribute to 4-methoxy-TEMPO-induced cytotoxicity, autophagy, and DNA damage in HepG2 cells. Archives of toxicology, 92(2), 717-728.

+ Open protocol

+ Expand

Calcium Signaling and ROS Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell culture reagents, including minimum essential medium (MEM) and fetal bovine serum (FBS), were purchased from Life Technologies (Carlsbad, CA). For [Ca²⁺]i imaging studies, 35-mm dishes with glass bottom coverslips were obtained from MatTek Corp (Ashland, MA). CaCl₂ was from EMD Millipore (Billerica, MA), and Fura-2 AM, Pluronic F127 and the reactive oxygen species (ROS)-sensitive dye, 2’,7’-dichlorofluorescin diacetate (H₂DCFDA) were purchased from Life Technologies. DAF2-DA, ATP (disodium salt), TNF-alpha, and all other chemicals, unless noted otherwise, were from Sigma (St. Louis, MO). VEGF₁₆₅ was purchased from R & D Systems (Minneapolis, MN). PP2 (a known Src inhibitor) was from Enzo (Farmingdale, NY), and U0126 (a known MEK and so ERK inhibitor) was purchased from Promega Corp. (Madison, WI). CLA isomers were obtained from Matreya, LLC (Pleasant Gap, PA).

Ampey A.C., Boeldt D.S., Clemente L., Grummer M.A., Yi F., Magness R.R, & Bird I.M. (2019). TNF-alpha Inhibits Pregnancy-Adapted Ca2+ Signaling in Uterine Artery Endothelial Cells. Molecular and cellular endocrinology, 488, 14-24.

+ Open protocol

+ Expand

Oxidative Stress Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A fluorogenic dye, 2’,7’-dichlorofluorescin diacetate (H2DCF-DA), Hoechst 33342 and Rhod-2 AM were from Molecular Probes (Eugene, Oregon, USA). Fura-2/AM, Isopropanol and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were from Sigma-Aldrich (St. Louis, Missouri, USA). Anti- phospho-p38 and p38, PKG antibodies were purchased from Santa Cruz (Dallas, Texas USA); anti-eNOS, anti-iNOS, Bcl-2, Bax, and ERK 1/2 antibodies were obtained from Invitrogen (Waltham, Massachusetts, USA); anti- sGCα1 and β-actin antibodies from Sigma-Aldrich (St. Louis, Missouri, USA); anti- phospho-JNK and JNK antibodies were purchased from R&D System (McKinley Place NE, Minneapolis, USA); anti-phospho ERK 1/2 antibody was from Cell Signaling (Danvers, Massachusetts, USA).

Lee M.L., Sulistyowati E., Hsu J.H., Huang B.Y., Dai Z.K., Wu B.N., Chao Y.Y, & Yeh J.L. (2019). KMUP-1 Ameliorates Ischemia-Induced Cardiomyocyte Apoptosis through the NO–cGMP–MAPK Signaling Pathways. Molecules, 24(7), 1376.

+ Open protocol

+ Expand

Synthetic Wastewater Exposure Test

Check if the same lab product or an alternative is used in the 5 most similar protocols

Synthetic wastewater (SW) containing nutrients was prepared in distilled water, based on the recipe provided in [44 (link)]. All chemicals were of analytical grade. This wastewater was used during the operation of aerobic and anaerobic bioreactors for biomass production.
The following PPCPs were added to the SW for the exposure test: sulfamethoxazole (CAS 723-46-6), carbamazepine (CAS 298-46-4), ibuprofen (CAS 15687-27-1), triclosan (CAS 3380-34-5). All these chemicals were of 99% purity and supplied by Sigma-Aldrich.
Micropollutants were dissolved in methanol and maintained in concentrated stock solution of 2000 mg/L and −20 °C until use.
The following reagents were purchased from Sigma-Aldrich and were used to determine enzymatic activities: monochlorobimane (MCB), 2′,7′-dichlorofluorescin diacetate (H2DCF-DA), reduced glutathione (GSH) and the solvent dimethyl sulfoxide (DMSO, 99.9%).
Cell damage was evaluated using Live/Dead Backlight Bacterial Viability staining purchased from Molecular Probes, Invitrogen.

Díaz-Cubilla M., Letón P., Luna-Vázquez C., Marrón-Romera M, & Boltes K. (2022). Effect of Carbamazepine, Ibuprofen, Triclosan and Sulfamethoxazole on Anaerobic Bioreactor Performance: Combining Cell Damage, Ecotoxicity and Chemical Information. Toxics, 10(1), 42.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!