Branched 60K PEI (Sigma 181978), Branched 2K PEI (Polysciences 06089), Linear 25K PEI (Polysciences 23966) and Linear 2K PEI (Polyscienes 24313) were diluted to 5.5mg/ml in PBS, pH’ed to 7.4 and stored away from direct light. Endotoxin free ovalbumin (OVA) was used throughout the study (Invivogen, vac-pova). OVA was diluted to 5mg/ml in endotoxin free water. All antibodies were used according to the manufacturer’s protocols. SIINFEKL- and E749–57-tetramers were obtained from the Emory NIH Tetramer Core Facility. TC-1 cells were generated in the laboratory of T-C. Wu (Johns Hopkins University, Baltimore, USA, tested to be mycoplasma-free) and maintained in RPMI supplemented with 10% FBS, 1% penicillin–streptomycin and 50ug/ml G41824 . B16F10 (ATCC) and CT26 cells (ATCC) were maintained in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin according to protocols from ATCC. Peptides used in this study are listed in Supplementary Table 4 . All murine peptides used in this study were synthesized at least 95% purity from Peptide 2.0. All human neoantigen peptides were provided by Dr. Catherine J Wu (Dana Farber Cancer Institute, Harvard Medical School, USA). Peptides were first dissolved in DMSO to 50 mg/ml and further diluted in endotoxin free water. Flow cytometry antibodies used in this study are listed in Supplementary Table 5 .
Branched 60k pei
Manufactured by Merck Group
Branched 60K PEI is a polyethylenimine (PEI) product with an average molecular weight of 60,000 Daltons and a branched structure. It is a versatile laboratory reagent commonly used in various applications, such as transfection and nanoparticle formation.
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Lab products found in correlation
2 protocols using branched 60k pei
1
Polyethylenimine-based Delivery of OVA and Neoantigens
2
Polyethylenimine-based Delivery of OVA and Neoantigens
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Branched 60K PEI (Sigma 181978), Branched 2K PEI (Polysciences 06089), Linear 25K PEI (Polysciences 23966) and Linear 2K PEI (Polyscienes 24313) were diluted to 5.5mg/ml in PBS, pH’ed to 7.4 and stored away from direct light. Endotoxin free ovalbumin (OVA) was used throughout the study (Invivogen, vac-pova). OVA was diluted to 5mg/ml in endotoxin free water. All antibodies were used according to the manufacturer’s protocols. SIINFEKL- and E749–57-tetramers were obtained from the Emory NIH Tetramer Core Facility. TC-1 cells were generated in the laboratory of T-C. Wu (Johns Hopkins University, Baltimore, USA, tested to be mycoplasma-free) and maintained in RPMI supplemented with 10% FBS, 1% penicillin–streptomycin and 50ug/ml G41824 . B16F10 (ATCC) and CT26 cells (ATCC) were maintained in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin according to protocols from ATCC. Peptides used in this study are listed in Supplementary Table 4 . All murine peptides used in this study were synthesized at least 95% purity from Peptide 2.0. All human neoantigen peptides were provided by Dr. Catherine J Wu (Dana Farber Cancer Institute, Harvard Medical School, USA). Peptides were first dissolved in DMSO to 50 mg/ml and further diluted in endotoxin free water. Flow cytometry antibodies used in this study are listed in Supplementary Table 5 .
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