3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt

AFB1 and AFM1 (95% purity) were provided by Pribolab (Qingdao, Shangdong, China), and a stock liquor of 200 μM was formulated by dissolving in dimethyl sulfoxide (DMSO) (Sigma−Aldrich, St. Louis, MO, USA). RPMI 1640 and fetal bovine serum (FBS) used for cell growth were from Gibco (Grand Island, NY, USA). The antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) and nonessential amino acids (NEAAs) were obtained from Life Technologies (Carlsbad, CA, USA). L-Glutamine was supplied by Sigma-Aldrich. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and phosphate buffer solution were provided by Beyotime Biotechnology (Shanghai, China).

Soy Lecithin and cholesterol were obtained from Avanti. PTX was obtained from Aladdin Chemical Co., Ltd. Sulfhydryl polyethylene glycol (SH‐PEG2000) and DSPE‐PEG‐FITC were purchased from Shanghai ToYong Bio Tech. Inc. Chloroauric acid trihydrate (HAuCl₄·3H₂O) was purchased from Dibo Biological Technology Co., Ltd. Hexadecyl trimethyl ammonium bromide (CTAB) Shanghai Mokai Biological Technology Co., Ltd. Enhanced BCA Protein Assay Kit, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT), and penicillin–streptomycin solution were purchased from Beyotime. 4′,6‐diamidino‐2‐phenylindole (DAPI), Dulbecco's modified Eagle medium (DMEM) medium with high glucose, and RPMI1640 medium were purchased from HyClone. 3,3′‐dioctadecyloxacarbocyanine perchlorate (DiO) was obtained from Protein Biological Technology Co., Ltd. (Nanjing, China). Alexa Fluor 647 anti‐CD9 antibody was purchased from Abcam. CD11c‐FITC, CD3‐FITC, CD4‐APC, CD8‐Percp‐Cy5.5, CD80‐APC, and CD86‐PE antibodies were purchased from BD Pharmingen. ELISA kits for interlekin‐6 (IL‐6), interferon‐γ (IFN‐γ), and tumor necrosis factor‐α (TNF‐α) were obtained from Multi Sciences Biotech, Co., Ltd. The other chemical reagents with analytical grade were obtained from Solarbio Life Sciences.