Hepatic fatty acid composition was measured at LIPID MAPS lipidomics core at the University of California (San Diego) using esterified and non-esterified (total) fatty acid panel. Briefly, liver tissues were homogenized and lipid fraction was extracted using modified Bligh Dyer liquid/liquid extraction method. The lipids were saponified and the hydrolyzed fatty acids were extracted using a liquid/liquid method. The extracted fatty acids were derivatized using Pentaflourylbenzylbromine (PFBB) and analyzed by gas chromatography (GC) using an Agilent GC/MS ChemStation. Individual analytes were monitored using selective ion monitoring (SIM). Analytes were monitored by peak area and quantified using the isotope dilution method using a deuterated internal standard and a standard curve.
Gc ms chemstation
Manufactured by Agilent Technologies
The GC/MS ChemStation is a comprehensive software package designed to control and acquire data from Agilent gas chromatography and mass spectrometry (GC/MS) systems. It provides a user-friendly interface for method development, data acquisition, analysis, and reporting.
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2 protocols using gc ms chemstation
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Hepatic Fatty Acid Composition Analysis
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Hepatic Fatty Acid Composition Analysis
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Hepatic fatty acid composition was measured at LIPID MAPS lipidomics core at the University of California (San Diego) using esterified and non-esterified (total) fatty acid panel. Briefly, liver tissues were homogenized and lipid fraction was extracted using modified Bligh Dyer liquid/liquid extraction method. The lipids were saponified and the hydrolyzed fatty acids were extracted using a liquid/liquid method. The extracted fatty acids were derivatized using Pentaflourylbenzylbromine (PFBB) and analyzed by gas chromatography (GC) using an Agilent GC/MS ChemStation. Individual analytes were monitored using selective ion monitoring (SIM). Analytes were monitored by peak area and quantified using the isotope dilution method using a deuterated internal standard and a standard curve.
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