Bond max polymer refine detection kit

Thirty-two biopsy specimens with a histologic diagnosis of lentigo were randomly selected from the database at Pinkus Dermatopathology Lab, Monroe, MI. Lesions were chosen based on a clinical suspicion of lentigo maligna (melanoma in situ) but were histologically lentigo senilis or lentigo with focal melanocytic hyperplasia.
Each tissue section was formalin fixed (Thermo Scientific, Waltham, MA), embedded in paraffin (Cardinal Health, Dublin, OH), and cut at 4 µm thick. They were mounted onto charged slides and placed in a drying oven for 45 minutes at 60°C. The sections were immunostained with anti-SOX-10 antibody (prediluted RTU, Cell Marque, Rocklin, CA), anti-HMB-45 antibody (pre-diluted RTU, Leica Biosystems, Buffalo Grove, IL), and anti-Melan-A antibody (pre-diluted RTU, Leica Biosystems, Buffalo Grove, IL), using an automated Leica Bond-Max Polymer Refine Detection Kit (Leica Biosystems, Buffalo Grove, IL). The total number of melanocytes stained with each immunohistochemical stain was counted by two independent observers (DRM and TH). An average count was obtained from the two independent readings.
ANOVA test was used to analyze the significance of difference between three immunohistochemical stains. A linear mixed effects model was also employed to test the difference in the count of melanocytes. A p-value less than 0.05 was considered statistically significant.