Chromatographic solvents were from Fisher Scientific (Loughborough, UK), and used without further purification. The NMR experiments were performed on a Bruker AMX600 NMR spectrometer (600 MHz for 1H, and 150 MHz for 13C) (BRUKER UK, Coventry, UK). MS analyses were conducted on a Xevo G2-S ASAP (Waters Ltd., Herts, UK) or LTQ Orbitrap XL 1 spectrometers (Thermo Fisher Scientific, Runcorn, UK). UV spectra were obtained on Analytik Jena Specord 210 spectrophotometer (Thermo Fisher Scientific, Runcorn, UK). The solid-phase-extraction (SPE) fractions were analyzed on a Dionex Ultimate 3000 UHPLC, coupled with a photodiode array (PDA) detector, using a Phenomenex Gemini-NX 5 U C18 column (150 × 4.6 mm, 5 μm, Phenomenex (Macclesfield, UK), and gradient solvent systems comprising MeOH (solvent B) (Loughborough, UK) and water (solvent A) (both contained 0.1% TFA, flow rate: 1 mL/min) were employed for method developments for preparative HPLC purification. The column temperature was set at 25 °C.
Gemini nx 5 u c18 column
Manufactured by Phenomenex
Sourced in United Kingdom
The Gemini-NX 5 μ C18 column is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a 5 μm particle size and a C18 stationary phase. The column is designed for general separation and analysis applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using gemini nx 5 u c18 column
1
Analytical Methods for Natural Product Characterization
2
Peptide Separation and Desalting for Mass Spectrometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The TMT-labeled peptide mixture was separated through reversed-phase (RP)-high-performance liquid chromatography (HPLC) on a Waters e2695 HPLC separation system. Separation was performed on a Gemini-NX 5u C18 column (250 mm × 3.0 mm, 110 Å) (Phenomenex). LC separation was performed as previously reported with a 97 min basic gradient with a flow rate of 0.4 ml/min (23 (link)). The separated peptides were combined into 15 fractions and frozen at −20 °C after lyophilization. The dried peptides were resolubilized with 0.5% acetic acid and desalted using C18 StageTips (24 (link)). Desalted peptides were dried with a Speed-Vac concentrator, stored at −20 °C, and resuspended in 0.1% formic acid (FA) immediately before LC–MS/MS.
3
HPLC Profiling of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An analytical Agilent 1260 Infinity was used. Reversed-phase chromatography was performed on a Phenomenex Gemini-NX 5 U C 18 column (250 x 4.6 mm). The column temperature was set at 25 o C. A variable wavelength UV-Vis detector was set at 220 nm, 254nm and 360nm. An elution gradient was used with solvent A (1% trifluoroacetic acid in water) and solvent B (1% trifluoroacetic acid in MeOH). The initial mobile phase composition was 70% of A and 30% B at 0 min, then linear gradient to 100% of B over 30 min and held at that composition for 5 min before to returning to start conditions and column equilibration at flow rate of 0.800 mL/min. The chromatograms were monitored as 220 nm, 254 nm and 360 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!