ThermoPol Reaction Buffer Pack, AMV Reverse Transcriptase and its reaction buffer, Bst 2.0 DNA Polymerase, HiScribe™ T7 High Yield RNA Synthesis Kit, Monarch RNA Cleanup Kit, were all purchased from New England Biolabs (Beijing, China). dNTP Set 100 mM Solutions, RNase-Free DNase I and Eco32 I were obtained from Thermo Scientific (Shanghai, China). TRNzol Universal Reagent, EndoFree Mini Plasmid Kit II and Universal DNA Purification Kit were acquired from TIANGEN (Bei-jing, China). TB Green Premix Ex Taq was bought from Takara Biotechnology (Dalian, China). HiScript II Q RT SuperMix for qPCR was purchased from Vazyme (Nanjing, China). Betaine was supplied by Sigma (USA). Diethyl pyrocarbonate (DEPC) treated water, oligonucleotides and pcDNA 3.1 recombinant plasmids containing the DNA fragment of target genes were acquired from Sangon Bio-technology (Shanghai, China). The sequences of oligonucleotides are listed in Table S1 . The real-time fluorescence measurements of the RT-LAMP reactions were performed using an Applied Biosystem 7500 Fast real-time PCR instrument.
Dntp set 100 mm solutions
Manufactured by Thermo Fisher Scientific
Sourced in China, United States
The DNTP Set 100 mM Solutions is a set of four individual solutions, each containing a specific deoxynucleotide triphosphate (dNTP) at a concentration of 100 mM. The four dNTPs included are dATP, dCTP, dGTP, and dTTP. This product is commonly used in various molecular biology and genetic research applications that require the availability of purified dNTP solutions.
Automatically generated - may contain errors
Lab products found in correlation
Bst 2.0 dna polymerase, by New England Biolabs (2 mentions)
Thermopol reaction buffer pack, by New England Biolabs (1 mentions)
Endofree mini plasmid kit 2, by Tiangen Biotech (1 mentions)
Diethylpyrocarbonate depc treated water, by Sangon (1 mentions)
Amv reverse transcriptase, by New England Biolabs (1 mentions)
Eco32i, by Thermo Fisher Scientific (1 mentions)
Monarch rna cleanup kit, by New England Biolabs (1 mentions)
Rnase free dnase 1, by Thermo Fisher Scientific (1 mentions)
Hiscript 2 q rt supermix for qpcr, by Vazyme (1 mentions)
Tb green premix ex taq, by Takara Bio (1 mentions)
Betaine, by Merck Group (1 mentions)
7500 fast real time pcr instrument, by Thermo Fisher Scientific (1 mentions)
Hiscribe t7 high yield rna synthesis kit, by New England Biolabs (1 mentions)
Universal dna purification kit, by Tiangen Biotech (1 mentions)
Trnzol universal reagent, by Tiangen Biotech (1 mentions)
Oligonucleotide, by Sangon (1 mentions)
Betaine solution, by Merck Group (1 mentions)
Mirneasy serum plasma advanced kit, by Qiagen (1 mentions)
Evagreen, by Biotium (1 mentions)
Mirneasy mini kit, by Qiagen (1 mentions)
2 protocols using dntp set 100 mm solutions
1
RT-LAMP Assay for Gene Detection
2
Comprehensive qRT-PCR Reagents and Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bst 2.0 DNA Polymerase (New England Biolabs, Ipswich, MA, USA) was purchased from New England Biolabs. Betaine solution (Sigma-Aldrich, St. Louis, MO, USA) and EvaGreen (Biotium, Fremont, CA, USA) were supplied by Merck. dNTP Set 100mM Solutions (Thermo Scientific, Waltham, MA, USA) were bought from Thermo Fisher Scientific. Diethyl pyrocarbonate (DEPC) treated deionized water was purified using an Elga "Purelab Option" Water System. A miRNeasy Mini Kit and miRNeasy Serum/Plasma Advanced Kit (QIAGEN, Hilden, Germany) were purchased from QIAGEN. The miRNAs were acquired from Shanghai GenePharma Co., Ltd. (Shanghai, China). Oligonucleotides including padlock probe, FIP, BIP and F3 were synthesized and purified by Sangon Biotech (Shanghai, China) and had the following sequences (Table 1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!