To assess the changes in implantation protein profiles in BBT-EVs as a result of BBT-MSC in vitro communication, pre-confluent 100 mm2 tissue culture dishes of BBT-9 and BBT-18 were cultured for 48 h in media supplemented with MSC-EVs isolated by SEC from stimulated or unstimulated MSC. Confluent 100 mm2 tissue culture dishes of eMSC or pbMSC were cultured for 48 h with or without IFN-τ stimulation (MBS1131960, Mybiosource) (1000 ng/mL). Then, EVs from the different eMSC or pbMSC lines were pooled separately. In addition, to assess uterine cytokines effect on BBT-EVs, pre-confluent 100 mm2 tissue culture dishes of BBT-9 and BBT-18 were cultured in media supplemented with Activin A (PHC9564, Thermo Fisher Scientific) (100 ng/mL), Activin A, and FSTL1 (10924H08H50, Thermo Fisher Scientific) (100 and 50 ng/mL, respectively) or without cytokines during 48 h. The experimental design is shown in Figure 5 . Bead-assisted flow cytometry was performed on the BBT-EVs obtained following the procedures described to analyze the implantation marker expression of MMP2 (MA1-772, Thermo Fisher Scientific); HSPH1 (PA5-77793, Thermo Fisher Scientific); TDGF1 (NB100-1597, Novus Biologicals), and PEG3 (TA343590, Acris-antibodies).
Ta343590
Manufactured by OriGene
TA343590 is a lab equipment product offered by OriGene. It is a device designed for specific laboratory functions. No further details can be provided in an unbiased and factual manner without extrapolation.
Automatically generated - may contain errors
2 protocols using ta343590
1
Implantation Protein Profiling in BBT-EVs
2
Exploring Implantation Markers in BBT-EVs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the changes in implantation protein pro les in BBT-EVs as a result of BBT-MSC in vitro communication, pre-con uent 100-mm 2 tissue culture dishes of BBT-9 and BBT-18 were cultured for 48h in media supplemented with MSC-EVs isolated by SEC from stimulated or unstimulated MSC. Con uent 100-mm 2 tissue culture dishes of eMSC or pbMSC were cultured for 48 h with or without IFN-τ stimulation (MBS1131960, Mybiosource) (1000 ng/ml). Then, EVs from the different eMSC or pbMSC lines were pooled separately. In addition, to assess uterine cytokines effect on BBT-EVs, pre-con uent 100mm 2 tissue culture dishes of BBT-9 and BBT-18 were cultured in media supplemented with Activin A (PHC9564, Thermo Fisher Scienti c) (100 ng/ml), Activin A and FSTL1 (10924H08H50, Thermo Fisher Scienti c) (100 and 50 ng/ml respectively) or without cytokines during 48h. The experimental design is shown in Fig. 1. Bead-assisted ow cytometry was performed to BBT-EVs obtained following the procedures besides described to analyze the implantation marker expression of MMP2 (MA1-772, Thermo Fisher Scienti c); HSPH1 (PA5-77793, Thermo Fisher Scienti c); TDGF1 (NB100-1597, Novus Biologicals) and PEG3 (TA343590, Acris-antibodies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!