Abi 7000 real time detection system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI 7000 real-time detection system is a laboratory instrument designed for real-time quantitative PCR (qPCR) analysis. The core function of the ABI 7000 is to perform sensitive and accurate detection and quantification of nucleic acid sequences in real-time.

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ABI 7000 (58 citations) Real-Time System (23 citations) ABI 7000 system (16 citations) ABI Real-Time System (2 citations) ABI Prism 7000 Real-Time detection system (2 citations) ABI Prism 7000 Real-Time PCR Detection System (2 citations)

2 protocols using abi 7000 real time detection system

Quantitative HBV DNA Extraction and RT-qPCR

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A 200 µL aliquot of serum was used to extract HBV DNA using a Hepatitis B Viral DNA Quantitative Fluorescence Diagnostic Kit (Sheng Xiang Bio Inc., Changsha, Hunan, China) as per the manufacturer’s protocol and quantified by real-time polymerase chain reaction (RT-qPCR) with an ABI 7000 real-time detection system (Applied Biosystems, Foster City, CA, USA).

Cai J., Wu W., Wu J., Chen Z., Wu Z., Tang Y, & Hu M. (2022). Prevalence and clinical characteristics of hepatitis B surface antigen-negative/hepatitis B core antibody-positive patients with detectable serum hepatitis B virus DNA. Annals of Translational Medicine, 10(1), 25.

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Quantitative HBV Biomarker Analysis

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Serum levels of HBsAg and HBeAg were measured via ELISA analysis using HBV S antigen and HBV E antigen diagnostic kits (Ke Hua Bio Inc., Shanghai, PR China), respectively. HBV DNA was extracted from 200 μL aliquots of serum using a Hepatitis B Viral DNA Quantitative Fluorescence Diagnostic Kit (Sheng Xiang Bio Inc., Changsha, Hunan, PR China), and quantified by real-time polymerase chain reaction (PCR) using an ABI 7000 real-time detection system (Applied Biosystems, Foster City, CA, USA).

Shen T., Wu W.M., Du W.H., Wang L., He L.G., Tan L., Wang Z., Chen R., Hu M, & Ren Y.P. (2016). Positive association between serum apolipoprotein M levels and hepatitis B virus DNA load in HBeAg-negative chronic hepatitis B. Lipids in Health and Disease, 15, 210.

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