The largest database of trusted experimental protocols

K405 50

Manufactured by Abcam

The K405-50 is a lab equipment product. It is a 50 mL centrifuge tube made of polypropylene material.

Automatically generated - may contain errors

2 protocols using k405 50

1

Fluorescent Immunohistochemistry for Paraffin Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry for paraffin sections was performed using an immunohistochemistry kit from Biovision (K405-50; Milpitas, CA) according to manufacturer's protocols. Fluorescence staining of the tissue sections after permeabilization with 0.2% Triton X-100 was carried out as previously reported. 28 Protocol details are mentioned in Supplementary Methods.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Autophagy and Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry for detection of the LC3 protein and the macrophage marker F4/80 was carried out using standard protocols. Briefly, unstained sections of lungs or tumors were deparaffinized and rehydrated, and then incubated in the peroxidase blocking reagent (BioVision cat #K405-50). Antigen retrieval was perfomed by boiling the sections in sodium citrate for 20 min. To decrease background staining, the slides were incubated for 1 h in the mouse on mouse blocking reagent (Vector Labs MKB-2213-1), followed by overnight incubation with the primary antibodies (LC3B Cell Signaling 3868S rabbit polyclonal, 1:300; or F4/80 Cell Signaling 70076S rabbit monoclonal, 1:200). Next day, the slides were washed and incubated with One-Step HRP polymer (BioVision cat #K405-50) for 30 min at RT. The slides were then washed several times and then incubated with the DAB chromogen for 10 min at RT, followed by several washing steps and quick counterstain with Hematoxylin. Slides were then mounted and visualized under the upright microscope (Nikon).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!