Maxisorb Immunoplates were coated with mAb 4G8 (epitope 17 to 24) (Covance, http://www.bioscience.co.uk/covance ) in carbonate buffer overnight. Plates were blocked with 5% milk powder in PBS-Tween and samples were applied. The detection antibody was an Aβ42 selective rabbit mAb BA3-9 (Covance) followed by biotinylated anti-rabbit IgG (Sigma). Total Aβ was visualised by addition of 1 mg/ml 4-nitrophenyl phosphate and optical density was read in a spectrophotometer at 405 nm. Absorbance was measured on a microplate reader at 405 nm and results were calculated by comparison to serial dilutions of synthetic Aβ1–42.
Ba3 9
Manufactured by Fortrea
The BA3-9 is a compact and versatile laboratory equipment designed for a range of applications. It features a durable construction and a user-friendly interface. The core function of the BA3-9 is to provide reliable performance in the laboratory environment.
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Lab products found in correlation
2 protocols using ba3 9
1
Quantitative ELISA for Amyloid-β42
2
Amyloid-beta 42 ELISA Assay
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Nunc Maxisorp immunoplates were coated with mAb 4G8 (epitope 17-24) (Covance) in carbonate buffer overnight. Plates were blocked with 5% milk powder in PBS-tween, and samples were applied. The detection antibody was an Aβ42 selective rabbit mAb BA3-9 (Covance) followed by biotinylated anti-rabbit IgG (Sigma-Aldrich). Total Aβ was visualized by addition of the substrate (pNPP) 1 ng/mL, diluted in diethanolamine buffer, and optical density was read in a spectrophotometer at 405 nm.
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