Sw1990

The five human pancreatic cancer cell lines Panc1, MIA PaCa-2, BxPC-3, AsPC-1 and SW1990 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Panc1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, ATCC) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (pen/strep). MIAPaCa-2 cells were kept in DMEM (ATCC) supplemented with 10% (v/v) FBS, 2.5% (v/v) horse serum, and 1% (v/v) pen/strep. BxPC-3, AsPC-1 and SW1990 were maintained in RPMI 1640 medium (ATCC) containing 10% (v/v) FBS and 1% (v/v) pen/strep. The additional three primary pancreatic cancer cell lines PaCaDD135, PaCaDD159 and PaCaDD185 were kindly provided by Dr. Felix Rueckert (Surgical Clinic Mannheim, University of Heidelberg, Mannheim, Germany). Culture medium for primary pancreatic cancer cells lines was assembled by mixing two parts of DMEM medium supplemented with 20% (v/v) FBS with one part of Keratinocyte-SFM. All cell lines were incubated at 37 °C in 5% CO₂.

The human pancreatic cancer cell lines PANC-1 and SW1990 were obtained from American Type Culture Collection (ATCC, Manassas, USA). The cells were cultured according to the standard protocols that were provided by the supplier. In brief, the PANC-1 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, USA), containing foetal bovine serum (FBS; Invitrogen) at a final concentration of 10%. SW1990 cells were maintained in ATCC-formulated Leibovitz’s L-15 medium (ATCC) supplemented with FBS at a final concentration of 10%. Pancreatic cancer-associated fibroblasts (CAFs) were kindly provided by Dr. Chao Qu from Shanghai Cancer Center (Shanghai, China).

All of the cell lines used in this study were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), including the cancerous AsPC-1, CFPAC-1, Capan-1, SW1990, PANC-1, SW1990, BxPC-3, and MIA-PaCa-2 cell lines and the non-cancerous HPDE6C7 cell line. All of the above-mentioned cells were cultured in 5% CO₂ at 37 °C in 100% humidity in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% antibiotic/antifungal solution (Biowest, Nuaillé, France).