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Chitosan-Based Magnetic Nanoparticles Synthesis

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Ferric chloride, ferrous chloride, glutaraldehyde (25%) and Span-80 were purchased from Xilong Chemical Company (Guangdong, China). Chitosan and acetic acid were purchased from Sinopharm Chemical Reagent, Co. (Shanghai, China). Petroleum ether, ethanol (95%), ammonium hydroxide and liquid paraffin were procured from Myrell Chemical Technology (Shanghai, China). Unless otherwise specified, all chemical reagents were of analytical grade.
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Synthesis of Magnetic Polymer Nanoparticles

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Iron chloride and Tween 80 were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). Ethylene glycol, trisodium citrate, and sodium acetate were purchased from Beijing Chemical Works (Beijing, China). Acrylic acid (AA, purity ≥ 98%) was supplied by Wulian Chemical Factory (Shanghai, China). Potassium persulfate was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). N, N′-methylenebisacrylamide was purchased from Junyao Albert Biotechnology Company (Beijing, China). Liquid paraffin was purchased from Tianjin Bodi Chemical Co., Ltd. (Tianjin, China). Span 80 was purchased from Xilong Chemical Co., Ltd. (Shantou, China). Hydroxylamine hydrochloride and O-phenanthroline Hydrate were obtained from Yingdaxigui Chemical Reagent Factory (Tianjin, China). Agarose, pentobarbital sodium, and sodium carboxylmethyl cellulose were purchased from Sigma Aldrich (USA). Embospheres were purchased from Biosphere Medical Inc. (USA). Iodixanol injections were supplied by GE Healthcare Ireland (Ireland). All the other solvents and reagents were of analytical grade and used as received without further purification otherwise noted.
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