Afm ntegra vita microscope

The samples were prepared for AFM as follows. Buffer solution was used to bring the filler concentration to 2.5 mg/mL. Before their use, filler samples were incubated for 40 min at 37 °C. For hydrolysates, an equal volume of the sample was taken from the reaction mixture after 5, 40 and 120 min of incubation at 37 °C. We also analyzed the purified sample of the HPC homogenate diluted 21 times by phosphate buffer after it was incubated for 40 min at 37 °C. Next, two μL of the sample was transferred to freshly cleaved mica and incubated for 5 min. The sample was then washed twice in a drop of distilled water (deionized by a type I Milli-Q system) for 30 s and air-dried. AFM imaging was performed with an AFM Ntegra-Vita microscope (NT-MDT, Russia) in noncontact (tapping) mode in air. The typical scan rate was 0.5–1 Hz. Measurements were carried out using cantilevers NSG03 with a resonance frequency of 47–150 kHz, ensuring a 10 nm tip curvature radius. The processing and presentation of the AFM images were performed using Nova software (NT-MDT, Russia) and Gwyddion 2.44 software (http://gwyddion.net/, Czech Republic).

AFM was as described in [37 (link)]. AFM imaging was carried out using an AFM Ntegra-Vita microscope (NT-MDT Spectrum Instruments, Moscow, Russia) in noncontact (tapping) mode in air. The typical scan rate was 0.5–1 Hz. Measurements were carried out using NSG03 cantilevers with a resonance frequency of 47–150 kHz and ensured a 10 nm tip curvature radius. The processing and presentations of AFM images were performed using Nova PX software (NT-MDT Spectrum Instruments, Moscow, Russia) and Gwyddion 2.44 software (http://gwyddion.net/, Jihlava, Czech Republic).